The in-situ crosslinked feather keratin-silica hybrid films were successfully prepared by solvent casting in the presence of glycerol and γ-glycidoxypropyltrimethoxysilane (KH560). The structure and properties of the feather keratin-silica hybrid films were characterized by means of FTIR，TG，SEM and contact angle tests. The effect of KH560 amounts on the mechanical properties，thermal stability and the surface and bulk hydrophobicity was investigated. The results showed that in thermal and alkaline processed conditions

KH560 reacted onto feather keratin chains through the amino-oxirane addition reaction. Simultaneously the methoxysilane groups (Si-O-CH3) hydrolyzed to form silanol groups (Si-OH) and the condensation reaction of the silanol group (Si-OH) would form Si-O-Si linkages to result in a crosslinked structure. The crosslinking treatment improved the mechanical strength，thermal stability and the surface and bulk hydrophobicity.