Novel supermacropore cryogel beads were prepared by combining the inverse-suspension and cryo-polymerization methods under frozen condition. The mean diameter of the beads was 234.1 mm

and the pore size was 10-50 mm. The cation-exchange cryogel beads were prepared by in-situ grafting of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) onto the pore surface of the cryogel beads. The biocompatibility of the cryogel beadswas measured. The adsorption of protein and Cu2+ on the cryogel beads was also discussed. The growth of E.coli was little affected when cryogel beads existed in the cultivation. The cryogel beads had stronger adsorption ability after grafting AMPSA

and the adsorption capacity of Cu2+ could reach 1.14 mmol·g-1. Meanwhile

the cryogel beads grafted with AMPSA had the ability of protein adsorption

and the adsorption capacity for lysozyme reached 54.5 mg·g-1. Such cryogel beads could be used in immobilization of microorganisms

separation of biomass components and adsorption of heavy metal ions.