Butanol production and sugar utilization were greatly improved via oxidoreduction potential (ORP) control strategy during batch acetone-butanol-ethanol (ABE) fermentation with less acids accumulation. Batch fermentations using mixed sugars of glucose and fructose were conducted by pumping sterile air into the fermentor to control ORP above-490

-460

-430 and-400 mV

respectively. When ORP was controlled above-460 mV

butanol production and total solvents reached 13.19 g·L-1 and 19.71 g·L-1

respectively

increased by 139.38% and 117.07% compared to the uncontrolled process. At the end of fermentation

residual sugars concentration dropped to 3.2 g·L-1 and sugar utilization was as high as 94.18%. The results showed that the ORP-control strategy significantly improved sugar utilization

especially for fructose

and butanol production from mixed sugars

indicating that ORP-control could show a stimulatory effect on ABE fermentation and would be an economically competitive strategy for improving fermentation efficiency.