High-solid anaerobic fermentation has the advantages

such as higher biogas yield per unit volume

more organic waste disposal

smaller water requirement and less energy consumption. However

the reactor is difficult to start and easy to acidize due to high concentration of feedstock. Using corn stalk as substrate

the performance of start-up process

different inocula and slurry recycling methods were studied during the start-up process for high solid anaerobic fermentation. The reactor system remained stable during the start-up phase with the combined method of feedstock feeding and slurry recycling. The cumulative biogas yields were 43.54 ml·(g TS)-1 with sludge as inoculum and 115.15 ml·(g TS)-1 with corn straw wet fermentation slurry as inoculum in 22 days. X-Ray diffraction (XRD) showed that the CrI was strongly influenced by the microflora

which showed that semicellulose was mainly dissolved. Fluorescent in situ hybridization (FISH) demonstrated that the methanogenic archaea exhibited an obvious difference in the two methane fermentation systems inoculated with sludge and corn straw biogas slurry

respectively.