In order to provide technical support for high-value utilization of bio-methane

the traditional screening method was combined with high-throughput sequencing to develop a new method for screening strains of desulfurization bacteria. Soil samples were taken from the environment rich in desulfurization bacteria; then the same samples were treated with enriched medium. High-throughput sequencing of samples were performed in the V6 region of 16S rDNA to analyze species content and abundance

alpha diversity and species structures of a single group. The average data utilization ratio of high-throughput sequencing was 99.177%

adequately reflecting the samples' species content and structures of the V6 region. The species composition of soil samples was rich

including some desulfurization bacteria

such as Pseudomonadales

Rhizobiales

Desulfuromonadales

Desulfobacterales and Acidithiobacillales. Species screened from enriched medium mainly contained Pseudomonadales and Rhizobiales

and lower species diversity was observed. The high-throughput sequencing technology provided a more selective method in bacteria screening and enhanced understanding of the samples' species content and structure before screening desulfurization bacteria.