Mesoporous TiO2 with adjustable pore diameter

high specific surface area and excellent biocompatibility has a good application prospect in biological molecules immobilization carriers. Mesoporous TiO2-500 were prepared as the stationary phase packing in High Performance Liquid Chromatograph by calcinating H2Ti2O5 at 500℃

and the TiO2 samples were characterized by X-ray diffraction (XRD)

N2 adsorption-desorption and field-emmission scanning electron microscopy (FESEM). The residence time under different pH of bovine serum albumin

myoglobin and lysozyme through the new chromatograph column were tested. Nearly all three kinds of proteins were immobilized at pH =4.7

and the order of residence time of three proteins was lysozyme >myoglobin > bovine serum albumin under the condition of pH=7.4 and 11. Adsorption of three kinds of proteins on the surface of the mesoporous TiO2-500 under atmospheric pressure was consistent with chromatographic results

and the order of the immobilization capacity of mesoporous TiO2-500 for three kinds of proteins was lysozyme > myoglobin >bovine serum albumin. Therefore

due to the significant difference in protein immobilization performance

the mesoporous TiO2 has a potential application in protein adsorption and separation

immobilizing enzyme and other fields.