气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为

王亮; 薛闯; 白凤武

doi:10.3969/j.issn.0438-1157.2013.10.033

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气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为

生物化学工程与技术 | 更新时间：2025-12-10

- 气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为
- Oscillation attenuation by gas stripping in continuous very high gravity ethanol fermentation by self-flocculating yeast
- 化工学报 2013年第10期页码：3725-3731
- 作者机构：
  
  大连理工大学生命科学与技术学院,辽宁,大连,116023
- 作者简介：
  
  王亮(1986-),男,博士研究生.
- 基金信息：
- DOI：10.3969/j.issn.0438-1157.2013.10.033
  中图分类号： 939.9
- 网络首发：2013-10-05，
  
  纸质出版：2013-10-05
- 稿件说明：
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王亮, 薛闯, 白凤武. 气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为[J]. 化工学报, 2013,(10):3725-3731.

WANG Liang, XUE Chuang, BAI Fengwu. Oscillation attenuation by gas stripping in continuous very high gravity ethanol fermentation by self-flocculating yeast[J]. CIESC Journal, 2013, (10): 3725-3731.
王亮, 薛闯, 白凤武. 气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为[J]. 化工学报, 2013,(10):3725-3731. DOI： 10.3969/j.issn.0438-1157.2013.10.033.

WANG Liang, XUE Chuang, BAI Fengwu. Oscillation attenuation by gas stripping in continuous very high gravity ethanol fermentation by self-flocculating yeast[J]. CIESC Journal, 2013, (10): 3725-3731. DOI： 10.3969/j.issn.0438-1157.2013.10.033.

摘要

超高浓度(very high gravity

VHG)连续乙醇发酵过程中的振荡行为会导致发酵终点的乙醇浓度振荡和降低

是VHG连续乙醇发酵面临的主要问题。研究表明

游离酵母Saccharomyces cerevisiae 4126和自絮凝酵母BHL01在VHG连续乙醇发酵过程中

生物量、残糖、乙醇、甘油等发酵参数都呈现130～145 h的周期性振荡

且絮凝酵母发酵体系的平均乙醇浓度和乙醇生产强度都明显高于游离酵母体系。在絮凝酵母VHG连续乙醇发酵过程中利用发酵尾气气提分离乙醇

酵母细胞振荡行为被明显弱化

达到拟稳态状态

并且使发酵液中的残糖浓度控制在0.1 g·L-1以下

平均乙醇浓度为110.87 g·L-1

乙醇产率达到2.99 g·L-1·h-1。因此

本研究为弱化VHG连续乙醇发酵中的参数振荡行为提供了新的技术手段。

Abstract

Process oscillation is the main challenge in continuous very high gravity(VHG)ethanol fermentation

which could result in oscillation and decrease of ethanol concentration.In the present study

during continuous VHG ethanol fermentation by free cell Saccharomyces cerevisiae 4126 and self-flocculating yeast BHL01

sustained process oscillations of biomass

residual glucose

ethanol and glycerol

were observed with a period of 130—145 h.Furthermore

in the self-flocculating yeast system

ethanol concentration and productivity were higher than those in the free yeast system.VHG oscillation was significantly attenuated and then a quasi-steady state was developed instead

when gas stripping with fermentative off-gas was used to separate ethanol during continuous VHG ethanol fermentation by self- flocculating yeast.Finally

the residual glucose kept below 0.1 g·L-1

and ethanol concentration and productivity were 110.87 g·L-1 and 2.99 g·L-1·h-1

respectively.The present study provides a new strategy for the attenuation of oscillation in continuous VHG ethanol fermentation.

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