2-Keto-L-gulonic acid (2-KGA)

the precursor for vitamin C synthesis

is produced by the mixed culture of Ketogulonicigenium vulgare and Bacillus megaterium.In this paper

the previously established kinetic model for 2-KGA mixed culture was firstly tested with the data of 80 industrial batches.Based on sensitivity analysis

it was found that some insensitive parameters might be assigned fixed values to minimize computing time.Then

the model was used to predict the most important state variables

i.e.

substrate and product concentrations.Moving data window technique and rolling parameter identification approach were used in the prediction process.4 h and 8 h ahead prediction errors for 2-KGA concentration were less than 5%.