In the present paper bamboo was hydrothermally treated to overcome biomass recalcitrance for enzymatic hydrolysis.The effects of pretreatment temperature

residence time

adding amount of cellulase and bamboo kinds on enzymatic hydrolysis of glucan/xylan were investigated.The result shows that hydrothermal treatment can significantly improve total monosaccharide yield.The optimal pretreatment temperature is 190℃ and residence time 10 min.Approximately 74.3% glucan and 54.0% xylan were transformed after 72 h enzymatic hydrolysis using 15 FPU·(g glucan)-1 of cellulose (about 1% of glucan)

which are 3.5-fold and 4.7-fold of untreated substrate respectively.Too high treating temperature and too long staying time can lead to degradation of xylose and glucose

so total yield of monosaccharide decreases.If cellulase amount adding increases from 15 FPU·(g glucan)-1 to 60 FPU·(g glucan)-1

the total sugar recovery goes up 21.5% and 9.9% for treated and untreated bamboo

respectively. To rise enzymatic digestibility of bamboo

it was more effective using hydrothermal pretreatment than increasing enzyme amount.Hydrothermal pretreatment was sensitive to raw biomass materials and significantly different results were obtained for different bamboo materials.