The glycerol production by fermentation in flasks by Candida glycerinogenes was studied. Dissolved oxygen（DO)was one of the most important factors in the cultivation of the strain by influencing cell growth and metabolites generated.Based on this result

batch fermentation of glycerol under diverse DO levels in a 5 L stirred fermentor was investigated.The metabolic flux analysis explained the phenomenon that sugar consumption was fast and glycerol synthesis was relatively less under low dissolved oxygen levels

mainly due to generated ethanol.Metabolic flux analysis also showed that HMP pathway played an important role in glycerol synthesis. Analysis of carbon metabolization and energy metabolization（oxidative phosphorylation and substrate level phosphorylation)of C.glycerinogenes in the process of glycerol fermentation showed that a high level of ATP synthesis at high dissolved oxygen levels to a certain degree inhibited substrate level phosphorylation so that more carbon flowed to glycerol synthesis. The intracellular redox balance （NADH/NAD+ ratio)reflected the competition between glycerol production and ethanol synthesis.