模拟胃肠道反应器的参数建立与冷模实验研究

张文龙; 詹晓北; 陈荦; 郑志永; 朱莉; 李志涛; 高敏杰

doi:10.11949/j.issn.0438-1157.20181488

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模拟胃肠道反应器的参数建立与冷模实验研究

生物化学工程与技术 | 更新时间：2026-07-24

- 模拟胃肠道反应器的参数建立与冷模实验研究
- Parameter estimation and cold model experiments of gastrointestinal simulation reactor
- 化工学报 2019年70卷第5期页码：1879-1886
- 作者机构：
  
  1.江南大学生物工程学院糖化学与生物技术教育部重点实验室，江苏无锡 214122
  2.江南大学环境与土木工程学院，江苏无锡 214122
  3.无锡格莱克斯生物科技有限公司，江苏无锡 214125
- 作者简介：
  
  张文龙（1994—），男，硕士研究生，bonapetite@163.com
  郑志永（1973—），男，博士，教授，zhiyong@jiangnan.edu.cn
- 基金信息：
  
  国家重点研发计划重点专项项目(2017YFD0400302)
- DOI：10.11949/j.issn.0438-1157.20181488
  中图分类号： TQ 027.6
- 收稿：2018-12-18，
  
  修回：2019-02-15，
  
  网络首发：2026-07-24，
  
  纸质出版：2019-05-05
- 稿件说明：
移动端阅览
张文龙, 詹晓北, 陈荦, 郑志永, 朱莉, 李志涛, 高敏杰. 模拟胃肠道反应器的参数建立与冷模实验研究[J]. 化工学报, 2019, 70(5): 1879-1886

ZHANG Wenlong, ZHAN Xiaobei, CHEN Luo, ZHENG Zhiyong, ZHU Li, LI Zhitao, GAO Minjie. Parameter estimation and cold model experiments of gastrointestinal simulation reactor[J]. CIESC Journal, 2019, 70(5): 1879-1886
张文龙, 詹晓北, 陈荦, 郑志永, 朱莉, 李志涛, 高敏杰. 模拟胃肠道反应器的参数建立与冷模实验研究[J]. 化工学报, 2019, 70(5): 1879-1886 DOI： 10.11949/j.issn.0438-1157.20181488.

ZHANG Wenlong, ZHAN Xiaobei, CHEN Luo, ZHENG Zhiyong, ZHU Li, LI Zhitao, GAO Minjie. Parameter estimation and cold model experiments of gastrointestinal simulation reactor[J]. CIESC Journal, 2019, 70(5): 1879-1886 DOI： 10.11949/j.issn.0438-1157.20181488.

摘要

体外消化和发酵实验紧密结合才能模拟完整消化道的功能，现有的模拟胃肠消化和大肠发酵的反应器结构各异且不通用，组合多个独立反应器则增加了实验的复杂程度和设备成本。集成了两类反应器的优势特征，开发了一种采用可编程逻辑控制器（PLC）的模拟胃肠道反应器（GSR），模拟了整体的胃肠道动态特征并与体内数据进行了比较。结果表明模拟胃肠道反应器既可准确模拟胃和小肠的蠕动、排空和混合等动态特征，又可提供肠道微生物的发酵环境。尾气排放和收集装置的设计实现了尾气快速排出和尾气体积的准确计量。模拟胃肠道反应器可为体外探索益生元对肠道微生物作用等研究提供帮助。

Abstract

In vitro

digestion and fermentation experiments should be closely combined to simulate the function of the intact gastrointestinal tract. However

the existing simulated reactors for gastrointestinal digestion and large intestinal fermentation have different structures and are not universal. Combining the separated reactors would increase the complexity and the cost of the experiments. Integrated the dominant features of both types of reactors

a gastrointestinal simulation reactor(GSR) using a programmable logic controller(PLC) was developed in the present work. The dynamic characteristics of overall gastrointestinal tract were simulated and compared to the data

in vivo

. It shows that the developed reactor can accurately simulate the dynamic characteristics of peristalsis

emptying and mixing in the stomach and small intestine

and provide a fermentation environment for intestinal microbes. The exhaust gas discharge and collection device was considerately designed

the exhaust gas was quickly discharged and the volume could be accurately measured. The gastrointestinal simulation reactor can help to explore the role of prebiotics in intestinal microbes

in vitro

关键词

Keywords

references

伍鹏 . 体外仿生鼠胃消化系统的构建及其应用的研究 [D ] . 厦门 : 厦门大学 , 2016 . Wu P . Study on development and application of in vitro biomimetic rat stomach digestive systems [D ] . Xiamen: Xiamen University , 2016 .

刘颖 , 易涛 , 宦娣 , 等 . 脂质制剂体外动态肠吸收模型的建立及评价 [J ] . 药学学报 , 2011 , 46 ( 8 ): 983 - 989 .

Liu Y , Yi T , Huan D , et al . Establishment and evaluation of a dynamic in vitro intestinal absorption model of lipid formulations [J ] . Acta Pharmaceutica Sinica , 2011 , 46 ( 8 ): 983 - 989 .

华慧颖 , 闵玉涛 , 宋彦显 , 等 . 淀粉体外消化模型的构建及在交联淀粉消化研究中的应用 [J ] . 食品工业 , 2011 , ( 7 ): 19 - 22 .

Hua H Y , Min Y T , Song Y X , et al . Formation of starch digestion model and digestibilityof cross-linking starch in vitro [J ] . The Food Industry , 2011 , ( 7 ): 19 - 22 .

邓人攀 . 基于仿生学原理的小肠模型反应器的设计和研究 [D ] . 厦门 : 厦门大学 , 2015 . Deng R P . Design and research of the small intestine model reactor based on bionics principles [D ] . Xiamen: Xiamen University , 2015 .

李志杰 . 基于人的体外模拟胃消化系统的构建及评价 [D ] . 杭州 : 浙江工商大学 , 2017 . Li Z J . Development and evaluation of human based artificial stomach digestion simulation system [D ] . Hangzhou: Zhejiang Gongshang University , 2017 .

Molly K , Vande Woestyne M , Verstraete W . Development of a 5-step multi-chamber reactor as a simulation of the human intestinal microbial ecosystem [J ] . Applied Microbiology and Biotechnology , 1993 , 39 ( 2 ): 254 - 258 .

Minekus M , Marteau P , Havenaar R , et al . A multicompartmental dynamic computer-controlled model simulating the stomach and small-intestine [J ] . Alternatives to Laboratory Animals , 1995 , 23 ( 2 ): 197 - 209 .

Minekus M , Smeets-Peeters M , Bernalier A , et al . A computer-controlled system to simulate conditions of the large intestine with peristaltic mixing, water absorption and absorption of fermentation products [J ] . Applied Microbiology and Biotechnology , 1999 , 53 ( 1 ): 108 - 114 .

Williams C F , Walton G E , Jiang L , et al . Comparative analysis of intestinal tract models [J ] . Annual Review of Food Science and Technology , 2015 , 6 : 329 - 350 .

Payne A N , Zihler A , Chassard C , et al . Advances and perspectives in in vitro human gut fermentation modeling [J ] . Trends in Biotechnology , 2012 , 30 ( 1 ): 17 - 25 .

Zheng Z Y , Chen Y Q , Zhan X B , et al . Mass transfer intensification in a novel airlift reactor assembly with helical sieve plates [J ] . Chemical Engineering Journal , 2018 , 342 : 61 - 70 .

刘明慧 , 邹超 , 肖杰 , 等 . 基于仿生学的柔性反应器 [J ] . 化工学报 , 2018 , 69 ( 1 ): 414 - 422 .

Liu M H , Zou C , Xiao J , et al . Soft-elastic bionic reactor [J ] . CIESC Journal , 2018 , 69 ( 1 ): 414 - 422 .

Maathuis A , Hoffman A , Evans A , et al . The effect of the undigested fraction of maize products on the activity and composition of the microbiota determined in a dynamic in vitro model of the human proximal large intestine [J ] . Journal of the American College of Nutrition , 2009 , 28 ( 6 ): 657 - 666 .

Elashoff J D , Reedy T J , Meyer J H . Analysis of gastric emptying data [J ] . Gastroenterology , 1982 , 83 ( 6 ): 1306 - 1312 .

李全阳 , 夏文水 . 市场流行搅拌型酸奶流变学特性的初步研究 [J ] . 食品工业科技 , 2003 , 24 ( 5 ): 43 - 47 . Li Q Y, Xia W S . A primary study on the rheological properties of stirred yoghourt [J ] . Science and Technology of Food Industry , 2003 , 24 ( 5 ): 43 - 47 .

咸娜 . 黄原胶复配及流变学特性和应用研究 [D ] . 上海 : 华东理工大学 , 2017 . Xian N . The rheological characteristics and application of xanthan gum and its hydrocolloids mixtures [D ] . Shanghai: East China University of Science and Technology , 2017 .

徐尧润 , 刘玉军 . 牛奶流变性能研究 [J ] . 中国乳品工业 , 1988 , 16 ( 5 ): 9 - 13 . Xu Y R, Liu Y J . Studies on rheologic properties of cow milk [J ] . China Dairy Industry , 1988 , 16 ( 5 ): 9 - 13 .

许威 . 菊粉物化特性的研究 [D ] . 洛阳 : 河南科技大学 , 2012 . Xu W . Study on physicochemical properties of inuiln [D ] . Luoyang: Henan University of Science and Technology , 2012 .

任妍娜 . 菊芋菊粉制取工艺优化及菊粉凝胶性质的研究 [D ] . 武汉 : 武汉工业学院 , 2011 . Ren Y N . Research about preparation process optimization and gel properties of inulin [D ] . Wuhan: Wuhan University of Technology , 2011 .

Kozu H , Kobayashi I , Nakajima M , et al . Mixing characterization of liquid contents in human gastric digestion simulator equipped with gastric secretion and emptying [J ] . Biochemical Engineering Journal , 2017 , 122 : 85 - 90 .

Nowak A , Slizewska K , Otlewska A . Antigenotoxic activity of lactic acid bacteria, prebiotics, and products of their fermentation against selected mutagens [J ] . Regulatory Toxicology and Pharmacology , 2015 , 73 ( 3 ): 938 - 946 .

Bellmann S , Lelieveld J , Gorissen T , et al . Development of an advanced in vitro model of the stomach and its evaluation versus human gastric physiology [J ] . Food Research International , 2016 , 88 : 191 - 198 .

Ehrlein H , Scheemann M . Gastrointestinal motility [DB/OL ] . Germany: Technische Universität München , 2005 . http://motility.humanbiology.wzw.tum.de/motvid01/tutorial.pdf http://motility.humanbiology.wzw.tum.de/motvid01/tutorial.pdf .

Smeets-Peeters M J , Minekus M , Havenaar R , et al . Description of a dynamic in vitro model of the dog gastrointestinal tract and an evaluation of various transit times for protein and calcium [J ] . Alternatives to Laboratory Animals , 1999 , 27 ( 6 ): 935 - 949 .

Bornhorst G M , Rutherfurd S M , Roman M J , et al . Gastric pH distribution and mixing of soft and rigid food particles in the stomach using a dual-marker technique [J ] . Food Biophysics , 2014 , 9 ( 3 ): 292 - 300 .

Marciani L , Gowland P A , Spiller R C , et al . Effect of meal viscosity and nutrients on satiety, intragastric dilution, and emptying assessed by MRI [J ] . American Journal of Physiology-Gastrointestinal and Liver Physiology , 2001 , 280 ( 6 ): G1227 -G 1233 .

Venema K . The TNO in vitro model of the colon (TIM-2) [M ] //Verhoeckx K , Cotter P , López-Expósito I , et al . The Impact of Food Bioactives on Health: In Vitro and Ex Vivo Models . Cham : Springer International Publishing , 2015 : 293 - 304 .

Venema K , Van Nuenen M , Smeets-Peeters M , et al . TNO's in vitro large intestinal model: an excellent screening tool for functional food and pharmaceutical research [J ] . Nutrition , 2000 , 24 ( 12 ): 558 - 564 .

Gasson M J , Davies F L . High-frequency conjugation associated with Streptococcus lactis donor cell aggregation [J ] . Journal of Bacteriology , 1980 , 143 ( 3 ): 1260 - 1264 .

Chiu Y T , Stewart M . Comparison of konjac glucomannan digestibility and fermentability with other dietary fibers in vitro [J ] . Journal of Medicinal Food , 2012 , 15 ( 2 ): 120 - 125 .

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