微波超声波联用萃取白藜芦醇及其苷的HPLC测定

马密霞; 秦宁; 闵清; 胡文祥

doi:10.11949/j.issn.0438-1157.20181483

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微波超声波联用萃取白藜芦醇及其苷的HPLC测定

分离工程 | 更新时间：2020-06-24

- 微波超声波联用萃取白藜芦醇及其苷的HPLC测定
- Resveratrol and polydatin by microwave-ultrasound extraction and determination by HPLC
- 化工学报 2019年第S1期页码：124-129
- 作者机构：
  
  1.武汉工程大学化学与环境工程学院, 湖北武汉 430073
  2.北京联合大学智慧城市学院, 北京 100101
  3.湖北科技学院药学院, 湖北咸宁 437100
  4.北京神剑天军医学科学院京东祥鹄微波化学联合实验室, 北京 101601
- 作者简介：
  
  马密霞（1967—），女，博士研究生，副教授，mixia107@163.com
  胡文祥（1961—），男，博士，教授，huwx66@163.com
- 基金信息：
  
  北京市创新基金项目(Z080104024508111)
- DOI：10.11949/j.issn.0438-1157.20181483
  中图分类号： R 284
- 收稿：2018-12-17，
  
  修回：2019-03-02，
- 稿件说明：
移动端阅览
马密霞, 秦宁, 闵清, 胡文祥. 微波超声波联用萃取白藜芦醇及其苷的HPLC测定[J]. 化工学报, 2019, 70(S1): 124-129

MA Mixia, QIN Ning, MIN Qing, HU Wenxiang. Resveratrol and polydatin by microwave-ultrasound extraction and determination by HPLC[J]. CIESC Journal, 2019, 70(S1): 124-129
马密霞, 秦宁, 闵清, 胡文祥. 微波超声波联用萃取白藜芦醇及其苷的HPLC测定[J]. 化工学报, 2019, 70(S1): 124-129 DOI： 10.11949/j.issn.0438-1157.20181483.

MA Mixia, QIN Ning, MIN Qing, HU Wenxiang. Resveratrol and polydatin by microwave-ultrasound extraction and determination by HPLC[J]. CIESC Journal, 2019, 70(S1): 124-129 DOI： 10.11949/j.issn.0438-1157.20181483.

摘要

建立了花生芽和葡萄籽微波超声波联用提取白黎芦醇及其苷的HPLC测定方法。采用高效液相色谱进行测定

色谱柱为迪马C

流动相为乙腈-水溶液，梯度洗脱，流速1.0 ml/min，柱温为30℃，检测波长为306 nm，进样量为20 μl。白黎芦醇在0.343～15.15×10

μg/ml范围内呈良好的线性关系，相关系数

= 0.9967；白藜芦醇苷在110.712～1774.23×10

μg /ml范围内线性关系良好，相关系数

= 0.9993。该方法检测快速、简便、重现性好，适用于花生芽和葡萄籽中白藜芦醇及其苷的定量分析。

Abstract

Objective to establish a method for the determination of resveratrol and polydatin in peanut bud and grape pip extract. High performance liquid chromatography (HPLC) was used. The chromatographic column was Di Ma C

the mobile phase was acetonitrile water solution

gradient elution

flow rate 1 ml/min

column temperature 30℃

306 nm and 20 μl. The linear range of resveratol showed good linear relationship 0.343－15.15×10

μg /ml

= 0.9967. The linear range of polydatin showed good linear relationship 110.712－1774.23×10

μg/ml

= 0.9993. The method is rapid

simple and reproducible

and is suitable for quantitative analysis of resveratrol in peanut sprouts and grape seeds.

关键词

Keywords

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