A new mixed-mode chromatography (MMC) adsorbent

named Adhere NUPharose FF with functional ligand N-benzyl-N-merhylethanolamine was prepared and used for removing the leaked Protein A ligand while purifying antibody. The ligand presents hydrophobic

electrostatic and hydrogen bond interactions between Adhere NUPharose FF and adsorbate. The agarose beads were activated by allyl glycidyl ether and conditions of the activation and processes of ligand coupling on the agarose beads were optimized

leading to an activated density of 260 μmol·ml-1 and a ligand density of 120 μmol·ml-1. Meantime

the mixture of antibody and Protein A was used to test the ability of the prepared adsorbent to remove Protein A from antibody. The data showed that it could remove 83.1% Protein A from antibody preparation

and supply feasible method to remove Protein A from antibody product.