The structure and catalytic stability of enzymes are of intriguing interest in biocatalysis and biotransformation. Compared with monomeric enzymes

the subunit self-assembling of oligomeric enzymes brings superior structural and functional properties. However

a series of problems also exist in the course of preparation and application of oligomeric enzymes

such as poor preparation efficiency

lower utilization of catalytic sites and less catalytic stability. Among them

the weak catalytic stability resulting from subunits dissociation severely limits the wider industrial applications of oligomeric enzymes. Currently

medium engineering

multi-subunit immobilization

subunit interface engineering and fusion protein strategy have been used to improve the oligomeric enzyme stability. Besides

the design strategy of transforming oligomeric enzymes into monomers attempts to solve the preparation and application problems fundamentally and has an extensive application prospect. In this paper

the desirable functions generated by oligomeric enzyme evolution were introduced first. Then

the preparation and application problems were summarized. At last

the strategies for improving oligomeric enzyme preparation and stabilization were reviewed.