Antibody

especially monoclonal antibody has become an important therapeutic drug which has a broad market demand and prospect. Nowadays the bottleneck of antibody production has shifted from antibody expression to downstream processing. The typical antibody purification process starts with protein A affinity chromatographic to capture the antibody and includes two subsequent polishing steps for impurity removal

such as ionic exchange

hydrophobic interaction or hydroxyapatite chromatography. There are some limitations restricting the development in antibody industry. Therefore

the researchers are committed to develop novel purification methods

including mixed-mode chromatography (MMC) and short peptide biomimetic chromatography which remedy the weakness of traditional methods. According to the recent advances in the purification of monoclonal antibody drugs

protein A affinity chromatography and the novel chromatographic methods and their applications in monoclonal antibody purification are reviewed in the present article.