Candida antarctica lipase B(CALB) is immobilized by entrapment in a hydrophobic silica xerogel. Firstly

the stability and reuse of immobilized enzymes are investigated and can be stored for at least 40 d at 25℃ without any significant loss of enzyme activity. Then

the transesterification of ethyl acetate with n-butanol in the presence of immobilized CALB is considered in a batch stirred tank reactor. The reaction kinetics is experimentally determined for different stirring rate

catalyst dosage

concentration and temperature. The suitable operating conditions are determined and a pseudo homogeneous kinetic model is established. The calculation data are in better agreement with the experimental data which shows that the kinetic equations are reasonable and suitable for simulation calculation.