Aiming at the process optimization of chromatographic separation

the high-throughput screening methods of protein adsorption with microtiter filter plate were established for initial resin screening

adsorption properties evaluation

adsorption isotherms and adsorption kinetics measurement

and adsorption and elution conditions optimization. Firstly

the operation parameters of 96-well filter plate were optimized

and with two ion exchangers and two mixed-mode resins as the model

the bovine serum albumin adsorption was investigated with different resins and liquid conditions by means of microtiter filer plate. The binding capacity maps were obtained and the appropriate adsorption-desorption conditions were determined. Further

the microtiter filter plate was applied to measure the adsorption isotherms and adsorption kinetics with four resins at specific adsorption conditions

and the adsorption parameters were obtained. Finally

the elution conditions were optimized using microtiter filter plate. The microscale results were comparable to that with packed-bed separation

indicating that the methods developed in the present work were practicable. The results demonstrated that the high-throughput process development of protein adsorption with microtiter filer plate was feasible and can be used to fast screen the best reins and liquid conditions. New methods showed the advantages of low resource consumption

large experimental fluxes

short developing period

wide application and high stability

which would be one new method for development of the protein separation process.