“Green” synthesis of bimetallic nanomaterials has drawn considerable attention in the field of nanotechnology. Au-Ag bimetallic nanomaterials were synthesized using extract of Artocarpus heterophyllus Lam leaves as reducing and capping agent. As a result of greater influence of temperature on nucleation and growth of Ag nanoparticles than the Au nanoparticles

Au-Ag alloys with a ratio of Au to Ag in 3:1 were obtained at 333 K

while alloys with a lower ratio of Au to Ag (1:1) were obtained at 363 K. This influence was illustrated by determining the nucleation and growth of Au and Ag nanoparticles using UV-Vis spectrophotometry method respectively

and it was also confirmed by FTIR analysis that only partial biomolecules were oxidized in the synthesis of Au-Ag (3:1) alloys. Au-Ag bimetallic nanoparticle with a ratio of Au to Ag in 1:1 generated a significant Raman enhancement in probing of Rhodamine 6G.