Affinity biomimetic chromatography is a novel bioseparation technology for the biological active substance

particularly showing good performance for antibody purification with the advantages of low cost

stable ligand structure

high specificity and so on. The critical point of affinity biomimetic chromatography is the specially-designed biomimetic ligand. The biomimetic ligands used currently include chemical synthetic ligand and short peptide ligand

which are normally obtained by rational design and screening. For a particular target protein

instead of selecting the ligand randomly

it is necessary to build a reasonable ligand library by rational designing method and screen the suitable mimetic ligand by high-throughput screening technology. According to the recent advances in affinity biomimetic chromatography

the designing and screening of ligands used for affinity mimetic chromatography and the applications in antibody purification are reviewed in the present article.