In the present paper

the bio-oil produced from hydrothermal liquefaction of D. tertiolecta was divided into n-hexane

chloroform and diethyl ether fractions via solvent division at first

and then chloroform fraction was purified by column chromatography. The n-hexane and diethyl ether fractions were directly analyzed by GC-MS and FT-IR

while the separation products obtained from column chromatography of chloroform fraction were combined with secondary mass spectrometry and FT-IR to confirm the product information. The results showed that chloroform fraction can be divided into sixteen components including the petroleum ether fraction (A1) mainly in alkenes

petroleum ether:ethyl acetate fraction (A2) mainly in carboxylic acid

petroleum ether:acetone fraction (A3) mainly in amides

petroleum ether:methanol fraction (A4) mainly in higher alkanes

and methanol fraction (A5) mainly in amides. In addition

the product recovery was reached up to 91.38% after the purification via column chromatography. Meanwhile

some components

which cannot be directly detected by GC-MS

were identified after column chromatography. The results gave a comprehensive insight in the compositions of product obtained from microalgal liquefaction

which provided the basis on further understanding of HTL mechanism and bio-oil upgrading.