The researches on organelles are important ways to study the structure and function of cells. Mitochondria

the principal energy-producing compartments in most cells

play roles in the numerous vital cellular processes. Thus

the organelles are crucially involved in various pathologies. As one of the most important cellular organelles

mitochondria are always the focus of researches. There are three kinds of mitochondrial fluorescence probes. This paper mainly introduces and discusses the progresses of mitochondrial staining kind of fluorescent probes in recent 10 years on the basis of the fluorophore with positive charge or the introduction of positioning groups such as triphenylphosphine.