Formation and structure control of macroporous microsphere is a complex process. Special techniques were developed for the preparation of gigaporous microspheres with large pore size over 100 nm. To meet the needs of gigaporous microsphere in biochemical engineering

the reverse micelles swelling method and the multiple emulsion method were developed which achieved the control of pore diameter above 100 nm. The effects of microsphere structures on their applications were also studied. When used in separation and purification of biomacromolecule

the gigaporous microspheres showed much higher capacity

activity recovery and purification fold. Immobilized enzymes in gigaporous microsphere also have some significant advantages in thermal stability

storage stability and reusability. The effective control of the microsphere structure is important for success application in different fields.