Metabolic engineering is an important development direction in the field of biology. So far it has a history of more than 20 years. Genetic modification of microorganisms by means of metabolic engineering can realize the sustainable production of a series of compounds

providing a potential solution for the energy and environmental problems. In recent years

some new technologies have been developed in the field of metabolic engineering

including the multivariable modular optimization technology

scaffold technology for enzyme assembly

dynamic control of metabolic flux and large-scale genome editing technology. Here we summarized and introduced the development and application of these technologies.