咪唑类离子液体在纤维素酶降解纤维素体系中的作用

范琳; 王少君; 李坤兰

doi:10.11949/j.issn.0438-1157.20141062

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咪唑类离子液体在纤维素酶降解纤维素体系中的作用

催化、动力学与反应器 | 更新时间：2025-12-10

- 咪唑类离子液体在纤维素酶降解纤维素体系中的作用
- Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose
- 化工学报 2015年第1期页码：121-125
- 作者机构：
  
  大连工业大学轻工与化学工程学院,辽宁,大连,116034
- 作者简介：
- 基金信息：
- DOI：10.11949/j.issn.0438-1157.20141062
  中图分类号： Q352.2
- 网络首发：2015-01-05，
  
  纸质出版：2015-01-05
- 稿件说明：
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范琳, 王少君, 李坤兰. 咪唑类离子液体在纤维素酶降解纤维素体系中的作用[J]. 化工学报, 2015,(1):121-125.

FAN Lin, WANG Shaojun, LI Kunlan. Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose[J]. CIESC Journal, 2015, (1): 121-125.
范琳, 王少君, 李坤兰. 咪唑类离子液体在纤维素酶降解纤维素体系中的作用[J]. 化工学报, 2015,(1):121-125. DOI： 10.11949/j.issn.0438-1157.20141062.

FAN Lin, WANG Shaojun, LI Kunlan. Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose[J]. CIESC Journal, 2015, (1): 121-125. DOI： 10.11949/j.issn.0438-1157.20141062.

摘要

以1-乙基-3-甲基咪唑磷酸二乙酯([Emim]DEP)、1-乙基-3-甲基咪唑醋酸([Emim]Ac)为研究对象

考察两种离子液体(IL)对酶法降解纤维素过程的影响。采用超声辅助离子液体溶解纤维素

[Emim]DEP和[Emim]Ac分别使纤维素的结晶度降为53.6%和62.3%。在纤维素酶降解再生纤维素的过程中

当IL的加入量小于2.0%时

对酶解过程起促进作用

其中加入量为0.5%时

促进作用最强

酶解率分别提高了11.4%和17.5%。在最优条件下(加入0.5%IL)

测量纤维素酶系中各个酶的酶活

结果表明离子液体对β-葡萄糖苷酶起到了明显的促进作用

分别使该酶酶活提高了120%和87%。离子液体降低了纤维二糖对葡聚糖外切酶的抑制作用

提高了酶解效率。

Abstract

Taking 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) and 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) as research targets

the influence of the two ionic liquids (IL) on the process of enzymatic degradation of cellulose was investigated. By using ultrasound to assist ionic liquids in dissolving cellulose

[Emim]DEP and [Emim]Ac made the crystallinity of cellulose decrease to 53.6% and 62.3% respectively. In the process of enzymatic degradation of regenerated cellulose

when the dosage of IL was less than 2.0%

IL acted as promoter

and when the dosage of IL was 0.5%

the promoting effect of IL was the strongest. Enzymatic hydrolysis rate increased by 11.4% and 17.5% respectively. Under the optimal conditions (add in 0.5% IL)

the enzyme activities of every enzyme in the cellulose system were measured. Ionic liquids had an obvious promoting effect on β-glucosidase

increasing enzyme activity by 120% and 87% respectively. Ionic liquids reduced inhibition of exoglucanase by cellubiose

and improved efficiency of enzymatic degradation of cellulose.

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