Amorphadiene

the role precursor of anti-malaria drug artemisinin

was produced by artificial Saccharomyces cerevisiae cells that were constructed under guidance of gene engineering methods. Homologous genes tHMGR and ERG20 as well as heterogenous gene ADS were integrated into yeast genome at the multiple copies Delta sites. Two advantages of this strategy were to increase copy number of genes and to enhance the stability of recombinant strains genotype. Overexpression of tHMGR and ERG20 genes increased accumulation of FPP

the common precursor for sesquiterpenoids. The artificial yeast strains could produce amorphadiene by introducing heterogenous gene ADS. The production of amorphadiene was increased to 225.3 mg·L-1 in 50 ml flask fermentation through optimizing fermentation condition. In 5 L fermentor

production of amorphadiene was increased to 1.05 g·L-1 after optimization of fermentation process and the fed-batch feeding strategy.