二乙胺/氯化铜预处理玉米秸秆强化酶解性能

濮阳鑫; 桂铮; 刘婷婷; 张雯雯; 杨文静; 杨荣玲; 贺爱永; 罗洪镇

doi:10.11949/0438-1157.20260647

您当前的位置：

首页 >

文章列表页 >

二乙胺/氯化铜预处理玉米秸秆强化酶解性能

生物化学工程与技术 | 更新时间：2026-07-08

- 二乙胺/氯化铜预处理玉米秸秆强化酶解性能
- Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency
- 化工学报 2026年
- 作者机构：
  
  1.淮安大学生命科学与食品工程学院，江苏淮安 223003
  2.淮阴师范学院化学化工学院，江苏淮安 223300
- 作者简介：
  
  濮阳鑫（2003—），女，硕士研究生，xpuyang@163.com
  罗洪镇（1988—），男，博士，副教授，hzluo@hyit.edu.cn
- 基金信息：
  
  国家自然科学基金项目(22578155);淮安市自然科学基金项目(HAB2024051);2026年江苏高校“青蓝工程”中青年学术带头人项目资助
- DOI：10.11949/0438-1157.20260647
  中图分类号： TQ 352
- 收稿：2026-05-11，
  
  修回：2026-07-07，
  
  录用：2026-07-08，
- 稿件说明：
移动端阅览
濮阳鑫, 桂铮, 刘婷婷, 等. 二乙胺/氯化铜预处理玉米秸秆强化酶解性能[J/OL]. 化工学报, 2026.

PUYANG Xin, GUI Zheng, LIU Tingting, et al. Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency[J/OL]. CIESC Journal, 2026. DOI: 10.11949/0438-1157.20260647.
濮阳鑫, 桂铮, 刘婷婷, 等. 二乙胺/氯化铜预处理玉米秸秆强化酶解性能[J/OL]. 化工学报, 2026. DOI： 10.11949/0438-1157.20260647.

PUYANG Xin, GUI Zheng, LIU Tingting, et al. Diethylamine/CuCl₂ pretreatment of corn stover for enhancing the enzymatic hydrolysis efficiency[J/OL]. CIESC Journal, 2026. DOI: 10.11949/0438-1157.20260647. DOI：

摘要

木质纤维素是最丰富的可再生资源，可为燃料和生物基化学品生产提供发酵原料，但利用效率严重受限于其复杂的致密结构，通常需要预处理破解木质纤维素的抗降解屏障，提高纤维素酶对底物的可及性。基于此，利用二乙胺-水体系预处理玉米秸秆，考察不同条件对秸秆组分和酶解性能的影响。结果表明，使用40%二乙胺和60%水作为共溶剂，在110℃下处理2 h后的木质素脱除率达86.4%，经酶解后的葡萄糖得率为99.3%。在此基础上，加入20 mM路易斯酸氯化铜可有效降低预处理中的反应压力，固体残渣中的葡聚糖含量增至47.7%，酶解后葡萄糖产量达26.6 g/100 g玉米秸秆。表征结果表明，二乙胺/氯化铜预处理能够破坏秸秆表面结构，促进木质素和半纤维素脱除，提高纤维素可及性和酶解效率。综上，该预处理体系将为木质纤维素高效利用提供技术借鉴。

Abstract

Lignocellulosic biomass is the most abundant renewable resource. It is mainly composed of cellulose

hemicellulose

and lignin

and the polysaccharide components can be hydrolyzed into fermentable sugars. The association between polysaccharides and lignin in lignocellulose gives rise to the recalcitrant structure that impedes efficient degradation. Therefore

developing efficient pretreatment to deconstruct its structure is of great significance for achieving comprehensive biomass utilization. Herein

using corn stover as the raw material

pretreatment with diethylamine/water was conducted to investigate the effects of pretreatment conditions on compositional changes and enzymatic hydrolysis. The results showed that using 40% diethylamine and 60% water as co-solvent to pretreat corn stover at 110°C for 2 h achieved the lignin removal of 86.4% and glucose yield of 99.3% was obtained after 72 h enzymatic hydrolysis. Furthermore

the effects of Lewis acid addition were investigated. With the addition of 20 mM CuCl

the pressure during pretreatment was effectively reduced and the glucan content in the solid fraction reached 47.7%

and the glucose production after pretreatment and enzymatic hydrolysis increased to 26.6 g/100 g corn stover. Some characterization techniques were used to elucidate the mechanisms of diethylamine-based pretreatment that enhances t

he enzymatic hydrolysis efficiency of corn stover. Characterization results revealed that the pretreatment disrupted the dense surface structure of corn stover

promoted the removal of lignin and hemicellulose

and improved cellulose accessibility and enzymatic hydrolysis efficiency. In summary

this proposed pretreatment system provides a technical reference for the efficient utilization of lignocellulosic biomass.

关键词

Keywords

references

Ren N Q , Zhao L , Chen C , et al . A review on bioconversion of lignocellulosic biomass to H 2 : Key challenges and new insights [J ] . Bioresource Technology , 2016 , 215 : 92 - 99 .

计宇轩 , 郑云 , 蒋永琪 , 等 . 生物炭基双金属催化剂的制备及其对左氧氟沙星的高效催化降解 [J ] . 南京工业大学学报(自然科学版) , 2025 , 47 ( 4 ): 459 - 468 .

Ji Y X , Zheng Y , Jiang Y Q , et al . Preparation of biochar-based bimetallic catalyst and its high efficiency catalytic degradation of levofloxacin [J ] . Journal of Nanjing Tech University (Natural Science Edition) , 2025 , 47 ( 4 ): 459 - 468 .

Long B , Zhang F Z , Dai S Y , et al . Engineering strategies to optimize lignocellulosic biorefineries [J ] . Nature Reviews Bioengineering , 2025 , 3 ( 3 ): 230 - 244 .

Mosier N , Wyman C , Dale B , et al . Features of promising technologies for pretreatment of lignocellulosic biomass [J ] . Bioresource Technology , 2005 , 96 ( 6 ): 673 - 686 .

Chen H Z , Liu Z H . Steam explosion and its combinatorial pretreatment refining technology of plant biomass to bio-based products [J ] . Biotechnology Journal , 2015 , 10 ( 6 ): 866 - 885 .

Verdía Barbará P , Choudhary H , Nakasu P S , et al . Recent advances in the use of ionic liquids and deep eutectic solvents for lignocellulosic biorefineries and biobased chemical and material production [J ] . Chemical Reviews , 2025 , 125 ( 12 ): 5461 - 5583 .

喻赛波 , 张雄 , 金勇 , 等 . 深度共熔溶剂选择性脱除烟梗木质素 [J ] . 化工学报 , 2026 , 77 ( 2 ): 726 - 737 .

Yu S B , Zhang X , Jin Y , et al . Delignification from tobacco stalks using deep eutectic solvents [J ] . CIESC Journal , 2026 , 77 ( 2 ): 726 - 737 .

Luo H Z , Zhou T R , Zhang R , et al . Conversion of biomass to biofuels: Integration of a ternary deep eutectic solvent pretreatment and microbial fermentation for C2-C4 bioalcohols production from lignocellulose [J ] . Industrial Crops and Products , 2024 , 220 : 119271 .

Lin K T , Wang C X , Guo M F , et al . Lignin with controlled structural properties by N-heterocycle-based deep eutectic solvent extraction [J ] . Proceedings of the National Academy of Sciences of the United States of America , 2023 , 120 ( 32 ): e2307323120 .

Xu L , Cao M F , Zhou J F , et al . Aqueous amine enables sustainable monosaccharide, monophenol, and pyridine base coproduction in lignocellulosic biorefineries [J ] . Nature Communications , 2024 , 15 : 734 .

Huang X Y , Fan M S , Xie J , et al . High titer ethanol production from poplar by aluminum chloride catalyzed organosolv pretreatment and simultaneous saccharification and fermentation [J ] . Industrial Crops and Products , 2022 , 181 : 114803 .

Wang J B , Zhou X , Zhang R , et al . N-heterocycle-based deep eutectic solvent-driven lignocellulosic biomass valorization: Efficient extraction of lignin facilitates enzymatic hydrolysis to produce bioethanol and butyric acid from corn stover [J ] . Renewable Energy , 2026 , 262 : 125361 .

Zhang D P , Liu J , Xu H X , et al . Improving saccharification efficiency of corn stover through ferric chloride-deep eutectic solvent pretreatment [J ] . Bioresource Technology , 2024 , 399 : 130579 .

Li W H , Tan X S , Miao C L , et al . Mild organosolv pretreatment of sugarcane bagasse with acetone/phenoxyethanol/water for enhanced sugar production [J ] . Green Chemistry , 2023 , 25 ( 3 ): 1169 - 1178 .

Liu S C , Shi T , He Z J , et al . Ethylenediamine pretreatment simultaneously improved carbohydrate hydrolysis and lignin valorization [J ] . Bioresource Technology , 2023 , 386 : 129552 .

Han X Y , Zhang X B , Dai T , et al . Enhancing the co-production of sugars from sugarcane bagasse via CuCl 2 -catalyzed organosolv pretreatment and additives [J ] . Fuel Processing Technology , 2023 , 241 : 107629 .

Zhao K , Zhao S S , Liu Q W , et al . Ternary deep eutectic solvent (T-CuCl 2 -EG) for lignin removal from corn straw [J ] . Journal of Biotechnology , 2026 , 409 : 195 - 201 .

Sun B X , Jiang H T , Jin G P , et al . Lewis acid-mediated choline chloride-glycerol deep eutectic solvent pretreatment for Goji berry branches: Enhancing enzymatic saccharification and high-value utilization of lignin [J ] . Biomass and Bioenergy , 2026 , 210 : 109072 .

Zhang C , Wang J B , Shao Y , et al . Utilization of N , N -dimethylethanolamine for deconstruction of lignocellulose by removing lignin to enhance enzymatic hydrolysis efficiency and microbial fermentability for bioalcohol production [J ] . ACS Sustainable Chemistry & Engineering , 2026 , 14 ( 19 ): 9328 - 9338 .

Zhao C B , Mai S Y , Fan M S , et al . Effects of metal chloride salt pretreatment and additives on enzymatic hydrolysis of poplar [J ] . Fermentation , 2023 , 9 ( 12 ): 1022 .

Sun B J , Zhang Y T , Li W P , et al . Facile synthesis and light-induced antibacterial activity of ketoprofen functionalized bacterial cellulose membranes [J ] . Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2019 , 568 : 231 - 238 .

Xu H F , Che X P , Ding Y , et al . Effect of crystallinity on pretreatment and enzymatic hydrolysis of lignocellulosic biomass based on multivariate analysis [J ] . Bioresource Technology , 2019 , 279 : 271 - 280 .

Vu A N , Nguyen N T , Nguyen T T , et al . Effect of bleaching process on the allomorph, crystalline index, morphology, and thermal stability of cellulose nanocrystals derived from rice straw [J ] . RSC Advances , 2026 , 16 ( 18 ): 16344 - 16357 .

Deng Y , Zhao Q S , Nian S , et al . Preliminary investigation into the use of amino-acid-derived ionic liquids for extracting cellulose from waste biomass to prepare cellulose aerogel adsorbents [J ] . Gels , 2025 , 11 ( 3 ): 210 .

Wang N , Xu B M , Wang X H , et al . Chemical and structural elucidation of lignin and cellulose isolated using DES from bagasse based on alkaline and hydrothermal pretreatment [J ] . Polymers , 2022 , 14 ( 14 ): 2756 .

Kukreti N , Kumar P , Kataria R . A sustainable synthesis of polyhydroxyalkanoate from stubble waste as a carbon source using Pseudomonas putida MTCC 2475 [J ] . Frontiers in Bioengineering and Biotechnology , 2024 , 12 : 1343579 .

Yao Z Y , Chong G , Guo H X . Deep eutectic solvent pretreatment and green separation of lignocellulose [J ] . Applied Sciences , 2024 , 14 ( 17 ): 7662 .

Madadi M , Liu D , Qin Y H , et al . Integrated pretreatment of poplar biomass employing p-toluenesulfonic acid catalyzed liquid hot water and short-time ball milling for complete conversion to xylooligosaccharides, glucose, and native-like lignin [J ] . Bioresource Technology , 2023 , 384 : 129370 .

Yang C R , Xing Z H , Zhou D Y , et al . Mild synergistic ethylenediamine-sodium carbonate pretreatment for high glucose yield and lignin degradation mechanism [J ] . Industrial Crops and Products , 2026 , 241 : 122825 .

Ma J X , Ma N L , Zhang W Q , et al . Highly efficient conversion of cellulose and hemicellulose using an innovative ternary deep eutectic solvent: Achieving the green and complete use of lignocellulose [J ] . Industrial Crops and Products , 2025 , 230 : 121091 .

He Y L , Xing Y K , Shao L P , et al . Enhancing enzymatic conversion of castor stalk through dual-functional ethanolamine pretreatment [J ] . International Journal of Biological Macromolecules , 2024 , 279 : 135293 .

Qin L , Li W C , Zhu J Q , et al . Ethylenediamine pretreatment changes cellulose allomorph and lignin structure of lignocellulose at ambient pressure [J ] . Biotechnology for Biofuels , 2015 , 8 ( 1 ): 174 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

温和条件下乙二醇苯醚高效分离回收甘蔗渣组分

生物转化脂肪酸合成ω-羟基酸和ω-氨基酸研究进展

超高压环境下离子液体微乳液预处理对水稻秸秆的影响

玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较

瞬间弹射蒸汽爆破增强离子液体对水稻秸秆的预处理效果