载酶海藻酸钙复合微球稳定水包油型Pickering乳液及其强化界面酶催化反应

王伟浩; 杨鑫; 李飞; 孙梦梦; 王垚磊; 孟涛

doi:10.11949/0438-1157.20190901

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载酶海藻酸钙复合微球稳定水包油型Pickering乳液及其强化界面酶催化反应

生物化学工程与技术 | 更新时间：2026-07-24

- 载酶海藻酸钙复合微球稳定水包油型Pickering乳液及其强化界面酶催化反应
- E@Alg@s-TiO₂ microsphere stabilized O/W Pickering emulsion and the enhancement of interfacial enzymatic catalysis
- 化工学报 2019年70卷第12期页码：4777-4786
- 作者机构：
  
  西南交通大学生命科学与工程学院，四川成都 610031
- 作者简介：
  
  王伟浩（1993—），男，硕士研究生，417216619@qq.com
  孟涛（1977—），男，博士，教授，taomeng@swjtu.edu.cn
- 基金信息：
  
  国家自然科学基金项目(21776230;21406181)
- DOI：10.11949/0438-1157.20190901
  中图分类号： TQ 032.4;Q 814.2
- 收稿：2019-08-06，
  
  修回：2019-09-05，
  
  网络首发：2020-06-09，
  
  纸质出版：2019-12-05
- 稿件说明：
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王伟浩, 杨鑫, 李飞, 孙梦梦, 王垚磊, 孟涛. 载酶海藻酸钙复合微球稳定水包油型Pickering乳液及其强化界面酶催化反应[J]. 化工学报, 2019, 70(12): 4777-4786

WANG Weihao, YANG Xin, LI Fei, SUN Mengmeng, WANG Yaolei, MENG Tao. E@Alg@s-TiO₂ microsphere stabilized O/W Pickering emulsion and the enhancement of interfacial enzymatic catalysis[J]. CIESC Journal, 2019, 70(12): 4777-4786 DOI: 10.11949/0438-1157.20190901.
王伟浩, 杨鑫, 李飞, 孙梦梦, 王垚磊, 孟涛. 载酶海藻酸钙复合微球稳定水包油型Pickering乳液及其强化界面酶催化反应[J]. 化工学报, 2019, 70(12): 4777-4786 DOI： 10.11949/0438-1157.20190901.

WANG Weihao, YANG Xin, LI Fei, SUN Mengmeng, WANG Yaolei, MENG Tao. E@Alg@s-TiO₂ microsphere stabilized O/W Pickering emulsion and the enhancement of interfacial enzymatic catalysis[J]. CIESC Journal, 2019, 70(12): 4777-4786 DOI: 10.11949/0438-1157.20190901. DOI：

摘要

以疏水改性二氧化钛修饰的载酶海藻酸钙微球（E@Alg@s-TiO

微球）稳定水包油（O/W）型Pickering乳液用于两相界面酶催化反应。与传统的两相游离酶体系相比，此体系以绿色、温和的方式将酶固定在乳液界面上，并强化了两相界面酶催化反应。研究成果归纳如下：油水比为1∶1.2时，Pickering乳液为O/W型；E@Alg@s-TiO

微球浓度为3%（质量）时稳定效果最好，脂肪酶的负载量为15.8 mg·g

-1

。以三丁酸甘油酯的水解反应为研究对象，该体系对油水体系的界面酶催化反应有很好的强化效果，具有96%转化率并提高酶活力7.8倍。重复使用5个批次能保留80%的酶活力。本研究进一步拓展了载酶海藻酸盐微球稳定的Pickering乳液体系的应用范围，有望为O/W体系的界面生物催化过程提供绿色平台。

Abstract

Hydrophobically-modified titanium dioxide-modified calcium alginate microspheres (E@Alg@s-TiO

microspheres) stabilized oil-in-water (O/W) type Pickering emulsion is used for two-phase interfacial enzyme catalysis. Compared with the traditional two-phase free enzyme system

the system immobilizes the enzyme at the emulsion interface in a green and gentle manner

and strengthens the two-phase interface enzyme catalyzed reaction. The research results are summarized as follows: when the oil-water ratio is 1∶1.2

the O/W emulsion is obtained; the concentration of the microspheres is 3%(mass). The O/W system has a good enhancement effect on the biphasic enzyme-catalyzed hydrolysis reaction

with 96% conversion rate and 7.8 times higher enzyme activity than the free enzyme in conventional two-phase system

5 batches cycles with 80% of residue enzyme activity. This study further expands the application range of the enzyme-loaded alginate microsphere-stabled Pickering emulsion system

which is expected to provide a green platform for the interfacial biocatalysis process.

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