SBBR反应器中耐冷微生物的驯化与识别

吴涵; 陈滢; 刘敏; 王淑莹; 张伟

doi:10.11949/0438-1157.20190731

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SBBR反应器中耐冷微生物的驯化与识别

能源和环境工程 | 更新时间：2026-07-24

- SBBR反应器中耐冷微生物的驯化与识别
- Domestication and identification of cold-resistant bacteria in SBBR reactor
- 化工学报 2020年71卷第2期页码：766-776
- 作者机构：
  
  1. 四川大学建筑与环境学院，四川成都 610065
  2. 北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京 100124
  3. 四川大学高分子研究所高分子材料工程国家重点实验室，四川成都 610065
- 作者简介：
  
  吴涵（1998—），女，本科生，1483112422@qq.com
  陈滢（1975—），女，副教授，cylm@163.com
- 基金信息：
  
  四川大学大学生创新训练计划(C2019104263);城镇污水深度处理与资源化利用技术国家工程实验室开放基金;2018年度中央高校基本科研业务费专项资金
- DOI：10.11949/0438-1157.20190731
  中图分类号： X 703
- 收稿：2019-07-01，
  
  修回：2019-07-31，
  
  网络首发：2020-08-14，
  
  纸质出版：2020-02-05
- 稿件说明：
移动端阅览
吴涵, 陈滢, 刘敏, 王淑莹, 张伟. SBBR反应器中耐冷微生物的驯化与识别[J]. 化工学报, 2020, 71(2): 766-776

WU Han, CHEN Ying, LIU Min, WANG Shuying, ZHANG Wei. Domestication and identification of cold-resistant bacteria in SBBR reactor[J]. CIESC Journal, 2020, 71(2): 766-776
吴涵, 陈滢, 刘敏, 王淑莹, 张伟. SBBR反应器中耐冷微生物的驯化与识别[J]. 化工学报, 2020, 71(2): 766-776 DOI： 10.11949/0438-1157.20190731.

WU Han, CHEN Ying, LIU Min, WANG Shuying, ZHANG Wei. Domestication and identification of cold-resistant bacteria in SBBR reactor[J]. CIESC Journal, 2020, 71(2): 766-776 DOI： 10.11949/0438-1157.20190731.

摘要

以模拟生活污水为处理对象，三种不同填料为生物膜载体，运行 A、B、C三个SBBR，通过逐步降温（25、20、15、10、6和5℃）培养驯化处理低温污水的生物膜。运行251周期，三个反应器COD出水浓度均低于50 mg/L；B反应器对氨氮去除效果优于A和C。特别在低温5℃时，A、B和C三个反应器氨氮平均出水浓度分别为14.1、3.79和14.1 mg/L。高通量测序结果显示，5℃时，三个反应器中均以降解有机物的微生物为主；只有B反应器中驯化富集出了嗜冷硝化菌（

Candidatus Nitrotoga

）；而其他有硝化作用的菌属在B反应器中的丰度也高于A和C反应器；不利于脱氮的类固氮菌（

Elstera

）在A和C反应器中丰度较高。此结果可从微生物角度解释三个反应器处理效果的差异性，表明B反应器处理低温污水有显著优势，其所用填料可结合低温菌进一步开发利用。

Abstract

Taking simulated domestic sewage as the treatment object

three different fillers are biofilm carriers

and three SBBRs of A

B and C are operated

and the biofilm of low temperature sewage is domesticated and treated by gradual cooling (25

6 and 5℃). During the 251 operating cycles

the COD effluent concentration of the three reactors was less than 50 mg/L. The ammonium removal efficiency of the B reactor was better than A and C. At 5℃

the ammonium concentration of effluent of A

B and C were 14.1

3.79 and 14.1 mg/L

respectively. The results of high-throughput sequencing showed that the dominant microbial species in SBBR were mainly

those can degrade organic matter at 5℃. Psychrophilic nitrifying bacteria (

Candidatus Nitrotoga

) were found only in B reactor. The abundance of other common nitrifying bacteria in the B reactor is higher than which in the A and C reactors. The nitrogen-fixing bacteria (

Elstera

)

which adverse to remove ammonium，has the minimal distribution in the B reactor. These results can explain the difference of the pollutant removal of the three reactors from point of view of microorganism

which shows that B reactor has significant advantages in treating low temperature wastewater.

关键词

Keywords

references

Cassidy D P , Belia E . Nitrogen and phosphorus removal from an abattoir wastewater in a SBR with aerobic granular sludge [J ] . Water Research , 2005 , 39 ( 19 ): 4817 - 4823 .

伍海全 , 周丽蓉 , 孙芬芬 , 等 . 应用生物强化技术处理高寒地区城市生活污水 [J ] . 环境工程学报 , 2017 , 11 ( 6 ): 3511 - 3517 .

Wu H Q , Zhou L R , Sun F F , et al . Application of biological enhancement technology on treating urban domestic sewage in alpine regions [J ] . Chinese Journal of Environmental Engineering , 2017 , 11 ( 6 ): 3511 - 3517 .

Antoniou P , Hamilton J , Koopman B , et al . Effect of temperature and pH on the effective maximum specific growth rate of nitrifying bacteria [J ] . Water Research , 1990 , 24 ( 1 ): 97 - 101 .

张巍 , 赵军 , 郎咸明 , 等 . 硝化细菌在不同温度下对氮素的去除效能研究 [J ] . 环境科学与管理 , 2010 , 35 ( 6 ): 83 - 86 .

Zhang W , Zhao J , Lang X M , et al . Study on nitrogen removal efficiency of nitrifying bacteria at different temperatures [J ] . Environmental Science and Management , 2010 , 35 ( 6 ): 83 - 86 .

王阿华 , 杨小丽 , 叶峰 . 南方地区污水处理厂低温生物脱氮对策研究 [J ] . 给水排水 , 2009 , 35 ( 10 ): 28 - 33 .

Wang A H , Yang X L , Ye F . Study on the countermeasures of low temperature biological nitrogen removal in wastewater treatment plants in Southern China [J ] . Water and Wastewater Engineering , 2009 , 35 ( 10 ): 28 - 33 .

刘海龙 , 任宇霞 , 张忠民 . 低温污水前置强化混凝 [J ] . 环境科学 , 2018 , ( 5 ): 2239 - 2248 .

Liu H L , Ren Y X , Zhang Z M . Enhanced coagulation as a pretreatment for low temperature wastewater [J ] . Environmental Science , 2018 , ( 5 ): 2239 - 2248 .

袁敏 , 刘晓冰 , 唐美珍 , 等 . 生物炭固定菌强化人工湿地对低温污水中氮素去除的模拟研究 [J ] . 生态与农村环境学报 , 2018 , 34 ( 5 ): 463 - 468 .

Yuan M , Liu X B , Tang M Z , et al . Study on removal of nitrogen from low temperature sewage by Pseudomonas flava WD-3 immobilized biochar in constructed wetland [J ] . Journal of Ecology and Rural Environment , 2018 , 34 ( 5 ): 463 - 468 .

Li G , Zhang Z . Anaerobic biological treatment of alginate production wastewaters in a pilot-scale expended granular sludge bed reactor under moderate to low temperatures [J ] . Water Environment Research , 2010 , 82 ( 8 ): 725 - 732 .

Tsushima I , Yoochatchaval W , Yoshida H , et al . Microbial community structure and population dynamics of granules developed in expanded granular sludge bed (EGSB) reactors for the anaerobic treatment of low-strength wastewater at low temperature [J ] . Journal of Environmental Science and Health , 2010 , 45 ( 6 ): 754 - 766 .

刘锁 , 牛川 , 李贺 . 低温下活性污泥处理废水技术的研究进展 [J ] . 环境科技 , 2019 , 32 ( 1 ): 58 - 62 .

Liu S , Niu C , Li H . Research progress in wastewater treatment by activated sludge at low temperature [J ] . Environmental Science and Technology , 2019 , 32 ( 1 ): 58 - 62 .

巩有奎 , 彭永臻 . 运行方式对SBBR亚硝酸型同步脱氮及N 2 O释放的影响 [J ] . 化工学报 , 2019 , 70 ( 6 ): 2289 - 2297 .

Gong Y K , Peng Y Z . Effect of operation mode on characteristics and N 2 O emissions during simultaneous nitrification and denitrification via nitrite in SBBR [J ] . CIESC Journal , 2019 , 70 ( 6 ): 2289 - 2297 .

Fuks G , Elgart M , Amir A , et al . Combining 16S rRNA gene variable regions enables high-resolution microbial community profiling [J ] . Microbiome , 2018 , 6 ( 1 ): 17 .

吴绍琳 , 刘文文 , 魏镇欢 , 等 . 不同时期排泥对培养SBBR生物膜的影响 [J ] . 化工进展 , 2019 , 38 ( 2 ): 1134 - 1139 .

Wu S L , Liu W W , Wei Z H , et al . Effects of sludge discharge stage on SBBR biofilm cultivation [J ] . Chemical Industry and Engineering Progress , 2019 , 38 ( 2 ): 1135 - 1140 .

Jin R C , Ma C , Yu J J . Performance of an Anammox UASB reactor at high load and low ambient temperature [J ] . Chemical Engineering Journal , 2013 , 232 : 17 - 25 .

艾胜书 , 张莉 , 王帆 , 等 . 多段多级AO生物膜反应器处理低温城市污水研究 [J ] . 水处理技术 , 2018 , 44 ( 7 ): 105 - 109 .

Ai S S , Zhang L , Wang F , et al . Study on low temperature municipal wastewater treatment by AMAO bio-film reactor [J ] . Technology of Water Treatment , 2018 , 44 ( 7 ): 105 - 109 .

张斌 , 刘波 , 罗雅文 , 等 . 改良型A 2 /O 工艺在高寒地区污水处理工程中的应用 [J ] . 给水排水 , 2015 , ( 12 ): 40 - 44 .

Zhang B , Liu B , Luo Y W , et al . Application of improved A 2 /O process in sewage treatment project in alpine region [J ] . Water and Wastewater Engineering , 2015 , ( 12 ): 40 - 44 .

Feng Y , Qi J , Chi L , et al . Production of sorption functional media (SFM) from clinoptilolite tailings and its performance investigation in a biological aerated filter (BAF) reactor [J ] . Journal of Hazardous Materials , 2013 , 246 / 247 ( 4 ): 61 - 69 .

寇海涛 , 曹文平 , 马天仪 , 等 . 污水处理系统中原生动物和微型后生动物的作用研究 [J ] . 工业安全与环保 , 2018 , 44 ( 5 ): 42 - 44 .

Kou H T , Cao W P , Ma T Y , et al . Functions analysis of protozoans and metazoans in wastewater bio-chemical treatment system [J ] . Industrial Safety and Environmental Protection , 2018 , 44 ( 5 ): 42 - 44 .

付欢 , 王欢 , 李杰 . 论钟虫对生化曝气池运行状况的指示作用 [J ] . 中国储运 , 2013 , ( 10 ): 172 - 173 .

Fu H , Wang H , Li J . Discussion on the indicative effect of vorticellidae on the operation status of biochemical aeration tank [J ] . China Storage and Transportation , 2013 , ( 10 ): 172 - 173 .

Lapinski J , Tunnacliffe A . Reduction of suspended biomass in municipal wastewater using bdelloid rotifers [J ] . Water Research , 2003 , 37 ( 9 ): 2027 - 2034 .

姚萌 , 李晔 , 张召基 . 低温条件下SBBR中污泥减量的对比研究 [J ] . 环境科学与技术 , 2008 , 31 ( 5 ): 98 - 100 .

Yao M , Li Y , Zhang Z J . Comparative study on sludge reduction of SBBR at low temperature conditions [J ] . Environmental Science and Technology , 2008 , 31 ( 5 ): 98 - 100 .

郑向阳 , 罗晓 , 袁立霞 , 等 . AO工艺处理淀粉污水效能及微生物群落解析 [J ] . 环境工程学报 , 2018 , 12 ( 3 ): 804 - 814 .

Zheng X Y , Luo X , Yuan L X , et al . Performance and bacterial community analysis of AO process treating starch wastewater [J ] . Chinese Journal of Environmental Engineering , 2018 , 12 ( 3 ): 804 - 814 .

Kragelund C , Nielsen J L , Thomsen T R , et al . Ecophysiology of the filamentous Alphaproteobacterium Meganema perideroedes in activated sludge [J ] . FEMS Microbiology Ecology , 2005 , 54 ( 1 ): 111 - 112 .

Horňák K , Kasalický V , Šimek K , et al . Strain-specific consumption and transformation of alga-derived dissolved organic ma tter by members of the Limnohabitans -C and Polynucleobacter -B clusters of Betaproteobacteria [J ] . Environmental Microbiology , 2017 , 19 ( 11 ): 4519 - 4535 .

Oh S , Choi D . Microbial community enhances biodegradation of bisphenol A through selection of sphingomonadaceae [J ] . Microbial Ecology , 2019 , 77 ( 3 ): 631 - 639 .

端正花 , 潘留明 , 陈晓欧 , 等 . 低温下活性污泥膨胀的微生物群落结构研究 [J ] . 环境科学 , 2016 , 37 ( 3 ): 1070 - 1074 .

Duan Z H , Pan L M , Chen X O , et al . Changes of microbial community structure in activated sludge bulking at low temperature [J ] . Environmental Science , 2016 , 37 ( 3 ): 1070 - 1074 .

Bowman J P , McCammon S A , Brown M V , et al . Diversity and association of psychrophilic bacteria in Antarctic sea ice [J ] . Applied and Environmental Microbiology , 1997 , 63 ( 8 ): 3068 - 3078 .

Rahalkar M , Bahulikar R A , Deutzmann J S , et al . Elstera litoralis gen. nov., sp. nov., isolated from stone biofilms of Lake Constance, Germany [J ] . Int . J . Syst . Evol . Microbiol . 2012 , 62 ( 8 ): 1750 - 1754 .

Pan K L , Gao J F , Li H Y , et al . Ammonia-oxidizing bacteria dominate ammonia oxidation in a full-scale wastewater treatment plant revealed by DNA-based stable isotope probing [J ] . Bioresource Technology , 2018 , 256 : 152 - 159 .

Tsuneda S , Miyauchi R , Ohno T , et al . Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene [J ] . Journal of Bioscience & Bioengineering , 2005 , 99 ( 4 ): 403 - 407 .

Alawi M , Lipski A , Sanders T , et al . Cultivation of a novel cold-adapted nitrite oxidizing betaproteobacterium from the Siberian Arctic [J ] . ISME Journal , 2007 , 1 ( 3 ): 256 - 264 .

Wang S , Peng Y , Ma B , et al . Anaerobic ammonium oxidation in traditional municipal wastewater treatment plants with low-strength ammonium loading: widespread but overlooked [J ] . Water Research , 2015 , 84 : 66 - 75 .

王晓慧 , 文湘华 , Craig Criddle , 等 . 城市污水处理厂活性污泥中氨氧化菌群落结构研究 [J ] . 环境科学 , 2009 , 30 ( 10 ): 3002 - 3006 .

Wang X H , Wen X H , Craig C , et al . Community structures of ammonia-oxidizing bacteria in activated sludge of wastewater treatment plant [J ] . Environmental Science , 2009 , 30 ( 10 ): 3002 - 3006 .

Igiehon N O , Babalola O O . Rhizosphere microbiome modulators: contributions of nitrogen fixing bacteria towards sustainable agriculture [J ] . Int . J . Environ . Res . Public Health , 2018 , 15 ( 4 ): 574 .

Sebastian L , Jasmin S , Christiane G D , et al . Nitrotoga-like bacteria are previously unrecognized key nitrite oxidizers in full-scale wastewater treatment plants [J ] . ISME Journal , 2015 , 9 ( 3 ): 708 - 720 .

韩梅 , 高伟 , 崔福义 . 对BAF预处理低温水源水系统中硝化细菌的识别 [J ] . 给水排水 , 2017 , 53 ( 9 ): 16 - 20 .

Han M , Gao W , Cui F Y . Identification of nitrifying bacteria in biological aerated filter in pretreatment of polluted raw water at low temperature [J ] . Water and Wastewater Engineering , 2017 , 53 ( 9 ): 16 - 20 .

Faith D P . Conservation evaluation and phylogenetic diversity [J ] . Biological Conservation , 1992 , 61 ( 1 ): 1 - 10 .

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