反硝化聚磷菌的培养富集及处理生活污水的稳定运行

王琦; 赵骥; 但琼鹏; 李夕耀; 张琼; 彭永臻

doi:10.11949/0438-1157.20190708

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反硝化聚磷菌的培养富集及处理生活污水的稳定运行

能源和环境工程 | 更新时间：2026-07-24

- 反硝化聚磷菌的培养富集及处理生活污水的稳定运行
- Cultivation and enrichment of denitrifying phosphorus removal system for treating domestic sewage
- 化工学报 2019年70卷第12期页码：4828-4834
- 作者机构：
  
  北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京市水质科学与水环境恢复工程重点实验室，北京 100124
- 作者简介：
  
  王琦（1995—），女，硕士研究生，wangqi19617@163.com
  彭永臻（1949—），男，教授，pyz@bjut.edu.cn
- 基金信息：
  
  北京市科技计划(D171100001017002);北京市教委资助项目
- DOI：10.11949/0438-1157.20190708
  中图分类号： X 703.1
- 收稿：2019-06-21，
  
  修回：2019-09-09，
  
  网络首发：2020-06-09，
  
  纸质出版：2019-12-05
- 稿件说明：
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王琦, 赵骥, 但琼鹏, 李夕耀, 张琼, 彭永臻. 反硝化聚磷菌的培养富集及处理生活污水的稳定运行[J]. 化工学报, 2019, 70(12): 4828-4834

WANG Qi, ZHAO Ji, DAN Qiongpeng, LI Xiyao, ZHANG Qiong, PENG Yongzhen. Cultivation and enrichment of denitrifying phosphorus removal system for treating domestic sewage[J]. CIESC Journal, 2019, 70(12): 4828-4834
王琦, 赵骥, 但琼鹏, 李夕耀, 张琼, 彭永臻. 反硝化聚磷菌的培养富集及处理生活污水的稳定运行[J]. 化工学报, 2019, 70(12): 4828-4834 DOI： 10.11949/0438-1157.20190708.

WANG Qi, ZHAO Ji, DAN Qiongpeng, LI Xiyao, ZHANG Qiong, PENG Yongzhen. Cultivation and enrichment of denitrifying phosphorus removal system for treating domestic sewage[J]. CIESC Journal, 2019, 70(12): 4828-4834 DOI： 10.11949/0438-1157.20190708.

摘要

在厌氧-好氧交替运行的序批式反应器（sequencing batch reactor

SBR）中，以C/P比大于50的实际生活污水为进水，成功驯化富集聚磷菌，平均厌氧释磷量为15 mg·L

-1

，出水

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842531&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842491&type=

6.68866634

3.47133350

-P浓度稳定小于0.5 mg·L

-1

。在系统运行的第74 d调整运行模式为厌氧-缺氧-好氧，在缺氧开始时短期投加

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842493&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842532&type=

5.50333357

3.47133350

-N配水以驯化培养反硝化聚磷菌。保持系统内

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842496&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842494&type=

5.50333357

3.47133350

-N浓度不变，在进水COD浓度为250 mg·L

-1

时，反硝化除磷效果最佳，平均反硝化除磷量占除磷量的比为87.1%。不同pH下反硝化除磷速率的小试证明，在pH=7.0时得到最大的比吸磷速率2.1 mg P·(g VSS·h)

-1

。此时调整

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842543&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842507&type=

5.50333357

3.47133350

-N进水为另一个全程硝化反应器的出水，并加大排水比增加缺氧初的进水量使得反应器内缺氧时的pH接近7.0，与未改变pH时对比表明前者在缺氧段反硝化除磷速率加快。反应器共运行160 d，稳定完成COD的去除与反硝化除磷过程。

Abstract

Phosphate accumulating organisms (PAOs) has been enriched successfully in an anaerobic-aerobic sequencing batch reactor (AO-SBR) with the influent of the domestic sewage with C/P ratio greater than 50.The average anaerobic phosphorus release amount was 15 mg·L

-1

. The concentration of effluent

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842546&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842539&type=

7.45066643

4.06400013

-P was less than 0.5 mg·L

-1

. On the 74 th day

the operation mode was adjusted to anaerobic-anoxic-aerobic

and

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842548&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842547&type=

6.26533318

4.06400013

-N was added at the beginning of anoxic zone to enrich the denitrifying phosphorus accumulating bacteria(DPB). The best efficiency of denitrifying phosphorus removal performance were acquired when the influent COD concentration was 250 mg·L

-1

and the

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842590&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842561&type=

6.26533318

4.06400013

-N concentration was constant in the system. Meanwhile the ratio of average denitrifying phosphorus removal to phosphorus removal was 87.1%. Anoxic pH effect tests showed that the rate of denitrifying phosphorus removal is related

to the pH value

DPB preferred higher pH for phosphorus removal

and the maximum specific phosphorus removal rate of 2.1 mg P·(g VSS·h)

-1

is obtained at pH=7.0. In the subsequent experiments

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842565&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=113842552&type=

6.26533318

4.06400013

-N was supplied from the effluent reactor and drainage ratio was increased. These methods ensured that the pH of the anaerobic reactor was closed to 7.0. The comparison with the unadjusted stage showed that the rate of denitrifying phosphorus removal increased. The reactor was operated for 160 days

and the COD removal and denitrifying phosphorus removal process were stably completed.

关键词

Keywords

references

Seviour R J , Mino T , Onuki M . The microbiology of biological phosphorus removal in activated sludge systems [J ] . FEMS Microbiology Reviews , 2010 , 27 ( 1 ): 99 - 127 .

Barnard J L . Biological nutrient removal without addition of chemicals [J ] . Water Research , 1975 , 9 ( 5 ): 485 - 490 .

Kuba T , Smolders G , van Loosdrecht M C M , et al . Biological phosphorus removal from wastewater by anaerobic-anoxic sequencing batch reactor [J ] . Water Science and Technology , 1993 , 27 ( 5 / 6 ): 241 - 252 ．

Saito T , Brdjanovic D , van Loosdrecht M C M . Effect of nitrite on phosphate uptake by phosphate accumulating organisms [J ] . Water Research , 2004 , 38 ( 17 ): 3760 - 3768 .

Vlekke G J F M , Comeau Y , Oldham W K . Biological phosphate removal from wastewater with oxygen or nitrate in sequencing batch reactors [J ] . Environmental Technology Letters , 1988 , 9 ( 8 ): 791 - 796 .

Meinhold J , Arnold E , Isaacs S . Effect of nitrite on anoxic phosphate uptake in biological phosphorus removal activated sludge [J ] . Water Research , 1999 , 33 ( 8 ): 1871 - 1883 .

Hu J Y , Ong S L , Ng W J , et al . A new method for characterizing denitrifying phosphorus removal bacteria by using three different types of electron acceptors [J ] . Water Research , 2003 , 37 ( 14 ): 3463 - 3471 .

李军 , 彭永臻 , 杨秀山 , 等 . 序批式生物膜法反硝化除磷特性及其机理 [J ] . 中国环境科学 , 2004 , 24 ( 2 ): 219 - 223 .

Li J , Peng Y Z , Yang X S , et al . Characteristics and mechanism of the phosphorus removal by denitrification with sequencing batch biofilm technique [J ] . China Environmental Science , 2004 , 24 ( 2 ): 219 - 223 .

Kuba T , van Loosdrecht M C M , Heijnen J J . Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system [J ] . Water Research , 1996 , 30 ( 7 ): 1702 - 1710 .

Kuba T , van Loosdrecht M C M , Brandse F A , et al . Occurrence of denitrifying phosphorus removing bacteria in modified UCT-type wastewater treatment plants [J ] . Water Research , 2016 , 31 ( 4 ): 777 - 786 .

Bortone G , Libelli S M , Tilche A , et al . Anoxic phosphate uptake in the dephanox process [J ] . Water Science and Technology , 1999 , 40 ( 4 / 5 ): 177 - 185 .

Whang L M , Park J K . Competition between polyphosphate and glycogen-accumulating organisms in enhanced-biological-phosphorus-removal systems: effect of temperature and sludge age [J ] . Water Environment Research , 2006 , 78 ( 1 ): 4 - 11 .

Wang Y Y , Peng Y Z , Li T W , et al . Phosphorus removal under anoxic conditions in a continuous-flow A 2 N two-sludge process [J ] . Water Science & Technology A Journal of the International Association on Water Pollution Research , 2004 , 50 ( 6 ): 37 - 44 .

Carvalho G , Lemos P C , Oehmen A , et al . Denitrifying phosphorus removal: linking the process performance with the microbial community structure [J ] . Water Research , 2007 , 41 ( 19 ): 4383 - 4396 .

Ma Y , Peng Y Z , Wang X L . Improving nutrient removal of the AAO process by an influent bypass flow by denitrifying phosphorus removal [J ] . Desalination , 2009 , 246 ( 1 / 2 / 3 ): 534 - 544 .

国家环境保护局 . 水和废水监测分析方法 [M ] . 4 版 . 北京 : 中国环境科学出版社 , 2002 .

The State Environmental Protection Administration . Water and Wastewater Monitoring and Analysis Method [M ] . 4th ed . Beijing: China Environmental Science Press , 2002 .

张勇 , 王淑莹 , 赵伟华 , 等 . 中试规模AAO曝气生物滤池双污泥系统的启动运行 [J ] . 化工学报 , 2015 , 66 ( 10 ): 4228 - 4235 .

Zhang Y , Wang S Y , Zhao W H , et al . Start-up of pilot-scale AAO-BAF two-sludge system [J ] . CIESC Journal , 2015 , 66 ( 10 ): 4228 - 4235 .

Randall C W , Bamard J L , Stensel H D . Design and Retrofit of Wastewater Treatment Plants for Biological Nutrient Removal: Volume V [M ] . CRC Press , 1998 .

王晓莲 , 王淑莹 , 彭永臻 . 进水C/P比对A 2 /O工艺性能的影响 [J ] . 化工学报 , 2005 , 56 ( 9 ): 1765 - 1770 .

Wang X L , Wang S Y , Peng Y Z . Effects of feed water C/P ratio on performance of anaerobic-anoxic-oxic process [J ] . Journal of Chemical Industry and Engineering(China) , 2005 , 56 ( 9 ): 1765 - 1770 .

Panswad T , Tongkhammak N , Anotai J . Estimation of intracellular phosphorus content of phosphorus-accumulating organisms at different P: COD feeding ratios [J ] . Journal of Environmental Management , 2007 , 84 ( 2 ): 141 - 145 .

郝王娟 , 薛涛 , 黄霞 . 进水磷碳比对聚磷菌与聚糖菌竞争生长的影响 [J ] . 中国给水排水 , 2007 , 23 ( 17 ): 95 - 98 .

Hao W J , Xue T , Huang X . Effect of influent P/C ratio on competition between phosphate and glycogen accumulating organisms [J ] . China Water and Wastewater , 2007 , 23 ( 17 ): 95 - 98 .

李志勇 , 彭永臻 , 张艳萍 , 等 . 硝酸盐浓度及投加方式对反硝化除磷的影响 [J ] . 环境污染与防治 , 2003 , 25 ( 6 ): 323 - 325 .

Li Z Y , Peng Y Z , Zhang Y P , et al . Effect of nitrate on denitrifying dephosphatation [J ] . Environmental Pollution and Control , 2003 , 25 ( 6 ): 323 - 325 .

Kerrn J P , Henze M . Biological phosphorus uptake under anoxic and aerobic conditions [J ] . Water Research , 1993 , 27 ( 4 ): 617 - 624 .

Kerrn-Jespersen J P , Henze M , Strube R . Biological phosphorus release and uptake under alternating anaerobic and anoxic conditions in a fixed-film reactor [J ] . Water Research , 1994 , 28 ( 5 ): 1253 - 1255 .

Wachtmeister A , Kuba T , van Loosdrecht M C M , et al . A sludge characterization assay for aerobic and denitrifying phosphorus removing sludge [J ] . Water Research , 1997 , 31 ( 3 ): 471 - 478 .

王亚宜 , 王淑莹 , 彭永臻 , 等 . 污水有机碳源特征及温度对反硝化聚磷的影响 [J ] . 环境科学学报 , 2006 , 26 ( 2 ): 186 - 192 .

Wang Y Y , Wang S Y , Peng Y Z , et al . The influence of carbon source and temperature on the denitrifying dephosphorus removal in wastewater treatment process [J ] . Acta Scientiae Circumstantiae , 2006 , 26 ( 2 ): 186 - 192 .

Kerrn-Jespersen J P , Henze M . Biological phosphorus uptake under anoxic and aerobic conditions [J ] . Water Research , 1993 , 27 ( 4 ): 617 - 624 .

王亚宜 , 杜红 , 彭永臻 , 等 . A 2 N反硝化除磷脱氮工艺及其影响因素 [J ] . 中国给水排水 , 2003 , 19 ( 9 ): 8 - 11 .

Wang Y Y , Du H , Peng Y Z , et al . A 2 N process for denitrifying phosphorus and nitrogen removal and its affecting factors [J ] . China Water and Wastewater , 2003 , 19 ( 9 ): 8 - 11 .

侯红勋 , 彭永臻 , 殷芳芳 , 等 . NO 2 - 作为电子受体对反硝化吸磷影响动力学研究 [J ] . 环境科学 , 2008 , 2 ( 7 ): 1874 - 1879 .

Hou H X , Peng Y Z , Yin F F , et al . Inhibitory effect kinetics of nitrite acting as electron acceptor on anoxic phosphorus uptake and denitrification [J ] . Environmental Science , 2008 , 2 ( 7 ): 1874 - 1879 .

马娟 , 彭永臻 , 王丽 , 等 . 反硝化除磷技术及其影响因素分析 [J ] . 工业水处理 , 2009 , 29 ( 4 ): 4 - 8 .

Ma J , Peng Y Z , Wang L , et al . Analysis of denitrification-dephosphorization technology and its influential factors [J ] . Industrial Water Treatment , 2009 , 29 ( 4 ): 4 - 8 .

任南琪 , 陈鸣歧 . pH值对反硝化除磷的影响 [J ] . 黑龙江科技学院学报 , 2004 , 14 ( 5 ): 284 - 286 .

Ren N Q , Chen M Q . Effect of pH on denitrification and phosphorus removal [J ] . Journal of Heilongjiang University of Science and Technology , 2004 , 14 ( 5 ): 284 - 286 .

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