黑曲霉作为分泌蛋白细胞工厂的研究进展

姚善泾; 蔡礼年; 林东强

doi:10.11949/0438-1157.20190591

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黑曲霉作为分泌蛋白细胞工厂的研究进展

综述与专论 | 更新时间：2026-07-24

- 黑曲霉作为分泌蛋白细胞工厂的研究进展
- Progress in Aspergillus niger as cell factory for secretory proteins
- 化工学报 2019年70卷第10期页码：3690-3703
- 作者机构：
  
  浙江大学教育部生物质化工重点实验室，化学工程与生物工程学院，浙江杭州 310027
- 作者简介：
  
  姚善泾（1957—），男，博士，教授，yaosj@zju.edu.cn
- 基金信息：
  
  国家自然科学基金项目(21576233;21878263);中央高校基本科研业务费专项资金
- DOI：10.11949/0438-1157.20190591
  中图分类号： TQ 028.8
- 收稿：2019-05-29，
  
  修回：2019-07-02，
  
  网络首发：2026-07-24，
  
  纸质出版：2019-10-05
- 稿件说明：
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姚善泾, 蔡礼年, 林东强. 黑曲霉作为分泌蛋白细胞工厂的研究进展[J]. 化工学报, 2019, 70(10): 3690-3703

YAO Shanjing, CAI Linian, LIN Dongqiang. Progress in Aspergillus niger as cell factory for secretory proteins[J]. CIESC Journal, 2019, 70(10): 3690-3703
姚善泾, 蔡礼年, 林东强. 黑曲霉作为分泌蛋白细胞工厂的研究进展[J]. 化工学报, 2019, 70(10): 3690-3703 DOI： 10.11949/0438-1157.20190591.

YAO Shanjing, CAI Linian, LIN Dongqiang. Progress in Aspergillus niger as cell factory for secretory proteins[J]. CIESC Journal, 2019, 70(10): 3690-3703 DOI： 10.11949/0438-1157.20190591.

摘要

黑曲霉具有极强的分泌蛋白的能力，其分泌的木质纤维素降解酶、淀粉酶、蛋白酶、脂肪酶等广泛应用于食品、饲料和生物技术等相关工业中。本文围绕黑曲霉生产同源和异源分泌蛋白，阐述黑曲霉作为分泌蛋白细胞工厂的巨大潜力。首先，通过总结黑曲霉表达系统的优越性，确定了黑曲霉作为分泌蛋白细胞工厂的开发前景。随后，介绍了初步构建黑曲霉分泌蛋白细胞工厂的一般流程。接着，总结了近十年来黑曲霉作为同源分泌酶细胞工厂的研究进展，提出在黑曲霉中定向表达分泌酶是深入研究黑曲霉自身分泌酶性质、功能和结构的理想策略。最后，总结了近年来黑曲霉作为异源分泌蛋白细胞工厂的研究进展，并从异源蛋白表达中遇到的难点出发介绍了完善黑曲霉细胞工厂来提高异源蛋白产量的对策。

Abstract

Aspergillus niger has a strong ability to secrete proteins

and its secreted lignocellulose degrading enzymes

amylases

proteases

and lipases are widely used in related industries such as food

feed

and biotechnology. We describe its great potential as cell factory for secretory proteins based on the production of homologous and heterologous secretory proteins by

A. niger

. Firstly

the prospect of

A. niger

as cell factory for secretory proteins is ascertained based on the superiority of its expression system. Then the standard procedure for construction of

A. niger

cell factory for secretory proteins is introduced. Subsequently

the advances on

A. niger

as cell factory for homologous secretases in the past decade are summarized. It is proposed that the directed expression of inherent secretases in

A. niger

is an ideal strategy to study their properties

functions and structures. Finally

the advances on

A. niger

as cell factory for heterologous secretory proteins in recent years are summarized

and based on the difficulties encountered in the expression of heterologous proteins

the countermeasures to

improve the production of heterologous proteins by further improvement of

A. niger

cell factory are introduced.

关键词

Keywords

references

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