冷冻保存中影响胞内冰生长的因素

诸凯; 谢艳琦; 王雅博

doi:10.11949/0438-1157.20190552

您当前的位置：

首页 >

文章列表页 >

冷冻保存中影响胞内冰生长的因素

流体力学与传递现象 | 更新时间：2020-06-24

- 冷冻保存中影响胞内冰生长的因素
- Factors affecting intracellular ice growth during cryopreservation
- 化工学报 2019年70卷第S2期页码：208-214
- 作者机构：
  
  天津商业大学天津市制冷技术重点实验室，天津 300134
- 作者简介：
  
  诸凯（1954—），男，博士，教授，zhukai210@tju.edu.cn
  王雅博（1984—），女，博士，讲师，wang_yabo@tjcu.edu.cn
- 基金信息：
  
  国家自然科学基金项目(51676139)
- DOI：10.11949/0438-1157.20190552
  中图分类号： TB 61
- 收稿：2019-05-22，
  
  修回：2019-06-08，
  
  纸质出版：2019-09-05
- 稿件说明：
移动端阅览
诸凯, 谢艳琦, 王雅博. 冷冻保存中影响胞内冰生长的因素[J]. 化工学报, 2019, 70(S2): 208-214

ZHU Kai, XIE Yanqi, WANG Yabo. Factors affecting intracellular ice growth during cryopreservation[J]. CIESC Journal, 2019, 70(S2): 208-214
诸凯, 谢艳琦, 王雅博. 冷冻保存中影响胞内冰生长的因素[J]. 化工学报, 2019, 70(S2): 208-214 DOI： 10.11949/0438-1157.20190552.

ZHU Kai, XIE Yanqi, WANG Yabo. Factors affecting intracellular ice growth during cryopreservation[J]. CIESC Journal, 2019, 70(S2): 208-214 DOI： 10.11949/0438-1157.20190552.

摘要

细胞胞内冰的形成会导致严重的细胞损伤从而导致低温贮存中的诸多问题。以蚕豆为研究对象，用细胞松弛素B溶解细胞骨架，使用低温显微系统在不同的冷却速率下进行冷冻实验。实验结果表明，使用细胞松弛素B处理过的细胞在冷冻过程中结晶温度更高，结晶时间更短，但细胞骨架对胞内冰的生长过程影响较小。外界条件起着关键作用，接种冰晶影响细胞内冰晶的形成温度及冰晶的生长速率。最后，通过光强度图对细胞的损伤程度进行了分析。

Abstract

The formation of intracellular ice of cells can cause severe cellular damage leading to many problems in cryopreservation. The broad bean was used as the research object

and the cytoskeleton was dissolved by cytochalasin B

and the freezing experiment was carried out at different cooling rates using a low temperature microscopic system. The experimental results show that the cells treated with cytochalasin B have higher crystallization temperature and shorter crystallization time during the freezing process

but the cytoskeleton has little effect on the growth process of intracellular ice. The external conditions play a key role

and the inoculation of ice crystals affects the formation temperature of ice crystals in the cells and the growth rate of ice crystals. Finally

the degree of damage to the cells was analyzed by light intensity map. It was found that the growth process of intracellular ice without cytoskeleton was similar to that of normal cells

but the crystallization rate was different

indicating that the cytoskeleton inside the cells affected the initiation of intracellular ice. Therefore

keeping the cytoskeleton intact before cryopreserving the plant sample can reduce the formation of intracellular ice and maintain cell morphology after rewarming.

关键词

Keywords

references

Fonseca S C , Gil L , Manso M C , et al . Modelling the influence of storage temperature and time after cutting on respiration rate of diced red onions ( Allium cepa L. cv. Vermelha da Póvoa) [J]. Postharvest Biology & Technology , 2018 , 140 ( C ): 27 - 33 .

Hoffmann N E , Bischof J C . The cryobiology of cryosurgical injury [J]. Urology , 2002 , 60 ( 2 -supp-S 1 ): 40 - 49 .

Phothiset S , Charoenrein S . Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues [J]. Journal of the Science of Food and Agriculture , 2014 , 94 ( 2 ): 189 - 196 .

Fujikawa S . Freeze-fracture and etching studies on membrane damage on human erythrocytes caused by formation of intracellular ice [J]. Cryobiology , 1980 , 17 ( 4 ): 351 - 362 .

Acker J P , Mcgann L E . Membrane damage occurs during the formation of intracellular ice [J]. Cryo Letters , 2001 , 22 ( 4 ): 241 - 254 .

Brown M S . Texture of frozen vegetables: effect of freezing rate on green beans [J]. J. Sci. Food Agr ., 1967 , 18 ( 2 ): 77 - 81 .

Alhamdan A , Hassan B , Alkahtani H , et al . Cryogenic freezing of fresh date fruits for quality preservation during frozen storage [J]. Journal of the Saudi Society of Agricultural Sciences , 2015 : S1658077 X 15300783 .

Hoffmann N E , Bischof J C . The cryobiology of cryosurgical injury [J]. Urology , 2002 , 60 ( 2 ): 40 - 49 .

Lagerveld B W . Cryosurgical induced injury of human cancerous tissues – How it works? [J]. British Journal of Medical & Surgical Urology , 2012 , 5 ( 5 ): S24 - S27 .

Ninagawa T , Eguchi A , Kawamura Y , et al . A study on ice crystal formation behavior at intracellular freezing of plant cells using a high-speed camera [J]. Cryobiology , 2016 , 73 ( 1 ): 20 - 29 .

Levitt J . Responses of Plants to Environmental Stresses: Vol 1: Chilling, Freezing and High Temperature Stresses [M]. 2nd ed . New York : Academic Press , 1980 .

Brown M S . Texture of frozen vegetables: effect of freezing rate on green beans [J]. Journal of the Science of Food & Agriculture , 2010 , 18 ( 2 ): 77 - 81 .

Barbosacanovas G V , Altunakar B , Mejialorio D . Freezing of fruits and vegetables. An agribusiness alternative for rural and semi-rural areas [J]. Fao Agricultural Services Bulletin , 2005 , 36 ( 9 ): 3911 - 3915 .

Mazur P . Physical factors implicated in the death of microorganisms at subzero temperatures [J]. Annals of the New York Academy of Sciences , 2010 , 85 ( 2 ): 610 - 629 .

Mazur P . Physical and chemical basis of injury in single-celled microorganisms subjected to freezing and thawing [J]. Annals of the New York Academy of Sciences , 1966 , ( 1 ): 213 - 315 .

Fennema O R , Powrie W D , Marth E H . Low-temperature Preservation of Foods and Living Matter [M]. New York : Marcel Dekker , 1973 .

Petzold G , Aguilera J M . Ice morphology: fundamentals and technological applications in foods [J]. Food Biophysics , 2009 , 4 ( 4 ): 378 - 396 .

Lozinsky V I , Plieva F M , Galaev I Y , et al . The potential of polymeric cryogels in bioseparation [J]. Bioseparation , 2001 , 10 ( 4/5 ): 163 - 188 .

Ware C B , Nelson A M , Blau C A . Controlled-rate freezing of human ES cells [J]. Biotechniques , 2005 , 38 ( 6 ): 879 - 880 .

Franco M , Hansen P J . Effects of hyaluronic acid in culture and cytochalasin B treatment before freezing on survival of cryopreserved bovine embryos produced in vitro [J]. In Vitro Cellular and Developmental Biology. Animal , 2006 , 42 ( 1/2 ): 40 - 44 .

Hosu B G , Mullen S F , Critser J K , et al . Reversible disassembly of the actin cytoskeleton improves the survival rate and developmental competence of cryopreserved mouse oocytes [J]. PLoS ONE , 2008 , 3 ( 7 ): 2787 - 2793 .

Shigehiko O , Tomoyuki F , Osato M . Electrical and rheological analysis of freezing injury of agricultural products [J]. International Journal of Food Properties , 2002 , 5 ( 2 ): 317 - 332 .

Orvar B L , Sangwan V , Omann F , et al . Early steps in cold sensing by plant cells: the role of actin cytoskeleton and membrane fluidity [J]. Plant Journal , 2010 , 23 ( 6 ): 785 - 794 .

Tuteja N , Gill S S . Abiotic stress response in plants: role of cytoskeleton [M]// Abiotic Stress Response in Plants. Wiley‐VCH Verlag GmbH & Co . KGaA , 2016 : 835 - 847 .

Kasper J C , Friess W . The freezing step in lyophilization: physico-chemical fundamentals, freezing methods and consequences on process performance and quality attributes of biopharmaceuticals [J]. European Journal of Pharmaceutics & Biopharmaceutics , 2011 , 78 ( 2 ): 248 - 263 .

张哲 , 郝俊杰 , 赵静 , 等 . 冷冻-复温过程中葡萄相变过程研究 [J]. 农业机械学报 , 2016 , 47 ( 9 ): 241 - 248 .

Zhang Z , Hao J J , Zhao J , et al . Study on the phase transition of grape in the process of freezing and rewarming [J]. Journal of Agricultural Machinery , 2016 , 47 ( 9 ): 241 - 248 .

杨戈尔 , 张爱丽 , 徐学敏 , 等 . 胞内冰晶形成(综述) [J]. 工程热物理学报 , 2007 , 28 ( z2 ): 55 - 57 .

Yang G E , Zhang A L , Xu X M , et al . Intracellular ice crystal formation (review) [J]. Journal of Engineering Thermophysics , 2007 , 28 ( z2 ): 55 - 57 .

Xu D , Wang H , Wang Y , et al . Ice crystal growth of living onion epidermal cells as affected by freezing rates [J]. International Journal of Food Properties , 2018 , 21 ( 1 ): 606 - 617 .

龚明 , 刘友良 . 接种冰晶对测定植物组织抗冻性的影响 [J]. 南京农业大学学报 , 1989 , 12 ( 2 ): 117 - 118 .

Gong M , Liu Y L . Effect of inoculation of ice crystals on the determination of frost resistance of plant tissues [J]. Journal of Nanjing Agricultural University , 1989 , 12 ( 2 ): 117 - 118 .

Guo X S . Function of polyamine catabolism and its main catabolic products in higher plants [J]. Chinese Bulletin of Botany , 2005 , 22 ( 4 ): 408 - 418 .

Schneider C A , Rasband W S , Eliceiri K W . NIH Image to ImageJ: 25 years of image analysis [J]. Nature Methods , 2012 , 9 ( 7 ): 671 - 675 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

水溶解CO2过程界面对流现象的PIV/LIF测量及传质系数预测

受限界面处流体分子行为的调控及相关分子热力学模型初探：基于高比表面氧化钛的研究进展

界面传质中Rayleigh对流的定量分析

Marangoni对流的纹影实验分析

气液传质过程中Marangoni对流的观测与定量分析