曝气强度对SBBR同步生物脱氮及N<sub>2</sub>O释放影响

巩有奎; 彭永臻

doi:10.11949/0438-1157.20190522

您当前的位置：

首页 >

文章列表页 >

曝气强度对SBBR同步生物脱氮及N₂O释放影响

能源和环境工程 | 更新时间：2020-06-24

- 曝气强度对SBBR同步生物脱氮及N₂O释放影响
- Effect of aeration intensity on simultaneous biological nitrogen removal and N₂O release from SBBR
- 化工学报 2019年70卷第11期页码：4410-4419
- 作者机构：
  
  1.烟台职业学院建筑工程系，山东烟台 264670
  2.北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室，北京 100124
- 作者简介：
  
  巩有奎（1977—），男，博士，副教授，260943813@qq.com
- 基金信息：
  
  国家自然科学基金项目(51508008);烟职博士科研项目(2018002)
- DOI：10.11949/0438-1157.20190522
  中图分类号： X 703.1
- 收稿：2019-05-19，
  
  修回：2019-06-15，
  
  纸质出版：2019-11-05
- 稿件说明：
移动端阅览
巩有奎, 彭永臻. 曝气强度对SBBR同步生物脱氮及N₂O释放影响[J]. 化工学报, 2019, 70(11): 4410-4419 DOI: 10.11949/0438-1157.20190522.

GONG Youkui, PENG Yongzhen. Effect of aeration intensity on simultaneous biological nitrogen removal and N₂O release from SBBR[J]. CIESC Journal, 2019, 70(11): 4410-4419 DOI: 10.11949/0438-1157.20190522.
巩有奎, 彭永臻. 曝气强度对SBBR同步生物脱氮及N₂O释放影响[J]. 化工学报, 2019, 70(11): 4410-4419 DOI: 10.11949/0438-1157.20190522. DOI：

GONG Youkui, PENG Yongzhen. Effect of aeration intensity on simultaneous biological nitrogen removal and N₂O release from SBBR[J]. CIESC Journal, 2019, 70(11): 4410-4419 DOI: 10.11949/0438-1157.20190522. DOI：

摘要

以生活污水为处理对象，采用碳纤维填料制成序批式生物膜反应器（sequencing batch biofilm reactor，SBBR），采用N

联合曝气的方式，通过改变N

和O

的比例，稳定系统内DO浓度为1.5 mg/L，考察不同曝气强度（30、20和10 L/h）下系统脱氮性能及N

O释放特性。异养菌和硝化菌共生于生物膜内，异养菌位于外层，硝化菌位于内层，曝气强度降低有利于外部异养菌大量增殖，生物膜厚度增加。曝气强度为30 L/h和10 L/h条件下，SBBR系统

http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=809826&type=

http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=809825&type=

11.76866722

3.47133350

去除率分别为95%以上和79.2%±1.6%，同步脱氮效率分别为46.2%±2.6%和62.1%±2.3%，N

O产率分别为6.25%±0.6%和2.93%±0.43%。缺氧阶段，反硝化过程和PHA（聚

–羟基烷酸酯）积累同时发生；好氧阶段，PHA呈先增加后减少的趋势。初始阶段增加的PHA为后续同步发生的反硝化过程提供了电子供体。AOB的好氧反硝化过程和异养菌反硝化过程均可导致N

O的产生。曝气强度降低导致水力剪切力下降，生物膜内缺氧范围扩大，缺氧区N

O停留时间延长，利于其反硝化减量。曝气强度由30 L/h降至10 L/h，微生物胞外聚合物（EPS）分泌减少，PS/PN（多糖/蛋白质）由8.59 mg/mg降至6.58 mg/mg

生物膜致密性降低，碳源和N

O以扩散形式进入缺氧区域能力增强，N

O释放量降低。

Abstract

Using domestic wastewater as influent and carbon fibre as biological carriers

the characteristics of SND(simultaneous nitrification and denitrification) process and N

O emission were studied under different aeration intensity (30

10 L/h)

with the DO(dissolved oxygen) concentration controlled at 1.5 mg/L by adjusting the ratio of N

and O

. Hetertrophic and nitrifying bacteria conexisted in the biofilm

which are located in the outer and inner layers of the biofilm

respectively. The decrease of aeration intensity was beneficial to the proliferation of the external heterotrophic bacteria. With the aeration intensity was controlled at 30 L/h and 10 L/h

the removal efficiency of

http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=809834&type=

http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=809830&type=

13.63133430

4.06400013

in the SBBR system were more than 95% and 79.2%±1.6%

the SND efficiency was 46.2%±2.6% and 62.1%±2.3%

the N

O yield was 6.25%±0.6% and 2.93%±0.43%

respectively. During the anoxic phase

the denitrification process and the accumulation of PHA (poly-

-hydroxyalkanoate) occurred simultaneously. In the following aerobic process

the PHA increased first and then decreased

which indicated that PHA could be used as internal carbon sources in the following denitrification process. Both the aerobic denitrification process of AOB and the denitrification process of denitrifying bacteria at the presence of lower oxygen led to the emissions of N

O. The decrease of aeration intensity leads to a decrease in hydraulic shearing force

an increase in the anoxic range in the biofilm

and an increase in the N

O residence time in the anoxic zone

which is beneficial to the denitrification reduction. As the aeration intensity decreased from 30 L/h to 10 L/h

the extracellular polymer (EPS) secretion decreased

and the PS/PN (polysaccharide/protein) decreased from 8.59 mg/mg to 6.58 mg/mg

resulting the reduction of the biofilm density

which created the favorable conditions for N

O transferring into the biofilm to complete the reduction process.

关键词

Keywords

references

Ravishankara A R , Daniel J S , Portmann R W . Nitrous oxide(N 2 O): the dominant ozone-depleting substance emitted in the 21st century [J]. Science , 2009 , 326 ( 5949 ): 123 - 125 .

Kong Q , Wang Z , Niu P , et al . Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process [J]. Bioresource Technology , 2016 , 210 : 94 - 100 .

Ye L , Ni B J , Law Y Y , et al . A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators [J]. Water Research , 2014 , 48 ( 1 ): 257 - 268 .

Kampschreur M J , Temmink H , Kleerebezem R , et al . Nitrous oxide emission during wastewater treatment [J]. Water Research , 2009 , 43 ( 17 ): 4093 - 4103 .

Wang J , Rong H , Zhang C . Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor [J]. Journal of Bioscience and Bioengineering , 2018 , 125 ( 5 ): 532 - 542 .

Zhao J , Feng L , Yang G , et al . Development of simultaneous nitrification- denitrification (SND) in biofilm reactors with partially coupled a novel biodegradable carrier for nitrogen-rich water purification [J]. Bioresource Technology , 2017 , 243 : 800 - 809 .

Han Y , Liu J , Guo X , et al . Micro-environment characteristics and microbial communities in activated sludge flocs of different particle size [J]. Bioresource Technology , 2012 , 124 ( 11 ): 252 - 258 .

Wen Q , Chen Z , Shi H . T-RFLP detection of nitrifying bacteria in a fluidized bed reactor of achieving simultaneous nitrification- denitrification [J]. Chemosphere , 2008 , 71 ( 9 ): 1683 - 1692 .

Stefano M , Giaime T , Giovannimatteo E . Evaluation of nitrous oxide gaseous emissions from a partial nitritation reactor operating under different conditions [J]. Desalination and Water Treatment , 2018 , DOI: 10.5004/dwt.2018.22934 http://dx.doi.org/10.5004/dwt.2018.22934 .

Todt D , Dorsch P . Mechanism leading to N 2 O production in wastewater treating biofilm systems [J]. Reviews in Environmental Science and Bio/Technology , 2016 , 15 ( 3 ): 355 - 378 .

Sabba F , Terada A , Wells G , et al . Nitrous oxide emissions from biofilm processes for wastewater treatment [J]. Applied Microbiology and Biotechnology , 2018 , 102 ( 22 ): 9815 - 9829 .

Kinh C , Suenaga T , Hori T , et al . Counter-diffusion biofilms have lower N 2 O emissions than co-diffusion biofilms during simultaneous nitrification and denitrification: Insights from depth-profile analysis [J]. Water Research , 2017 , 124 ( 1 ): 363 - 371 .

He Q , Chen L , Zhang S . Hydrodynamic shear force shaped the microbial community and function in the aerobic granular sequencing batch reactors for low carbon to nitrogen (C/N)municipal wastewater treatment [J]. Bioresource Technology , 2019 , 271 ( 1 ): 48 - 58 .

Liu Y , Tay J H . The essential role of hydrodynamic shear force in the formation of biofilm and granular sludge [J]. Water Research , 2002 , 36 ( 7 ), 1653 - 1665 .

He Q , Chen L , Zhang S . Simultaneous nitrification, denitrification and phosphorus removal in aerobic granular sequencing batch reactors with high aeration intensity: impact of aeration time [J]. Bioresource Technology , 2018 , 263 : 214 - 222 .

郭海燕 , 郭祯 , 柳志刚 , 等 . 不同曝气强度下SBMBBR和SBR脱氮除磷性能对比研究 [J]. 环境科学学报 , 2012 , 32 ( 3 ): 568 - 576 .

Guo H Y , Guo Z , Liu Z G , et al . Characteristics of nitrogen and phosphorus removal in SBR and SBMBBR with different aeration rates [J]. Acta Scientiae Circumstantiae , 2012 , 32 ( 3 ): 568 - 576 .

APHA(American Public Health Association) . Standard Methods for the Examination of Water and Wastewater [M]. Baltimore : Port City Press , 1998 .

Oehmen A , Keller-Lehmann B , Zeng R J , et al . Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems [J]. Journal of Chromatography A , 2005 , 1070 ( 1/2 ): 131 - 136 .

张建华 , 彭永臻 , 张淼 , 等 . 同步硝化反硝化SBBR处理低C/N比生活污水的启动与稳定运行 [J]. 化工学报 , 2016 , 67 ( 11 ): 4817 - 4824 .

Zhang J H , Peng Y Z , Zhang M , et al . Start-up and steady operation of simultaneous nitrification and denitrification in SBBR treating low C/N ratio domestic wastewater [J]. CIESC Journal , 2016 , 67 ( 11 ): 4817 - 4824 .

Yang Q , Liu X H , Peng C Y , et al . N 2 O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method [J]. Environmental Science & Technology , 2009 , 43 ( 24 ): 9400 - 9406 .

Gong Y K , Wang S H , Wang S , et al . Nitrous oxide production from sequencing batch reactor sludge under nitrifying conditions: effect of nitrite concentrations [J]. Environmental Technology , 2012 , 33 ( 4 ): 401 - 408 .

王荣昌 , 肖帆 , 赵建夫 . 生物膜厚度对膜曝气生物膜反应器硝化性能的影响 [J]. 高校化学工程学报 , 2015 , 29 ( 1 ): 151 - 158 .

Wang R C , Xiao F , Zhao J F . Effects of biofilm thickness on nitrification performance of membrane-aerated biofilm reactors [J]. Journal of Chemical Engineering of Chinese Universities , 2015 , 29 ( 1 ): 151 - 158 .

Bradford M M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry , 1976 , 72 ( 1 ): 248 - 254 .

Dubois M , Gilles K A , Hamilton J K , et al . Colorimetric method for determination of sugars and related substances [J]. Analytical Chemistry , 1956 , 28 ( 3 ): 350 - 356 .

赵青 , 卞伟 , 李军 , 等 . DO/NH 4 + -NN实现短程硝化过程中生物膜特性 [J]. 环境科学 , 2018 , 39 ( 3 ): 1278 - 1285 .

Zhao Q , Bian W , Li J , et al . Characteristics of biofilm during the transition process of complete nitrification and partial nitrification [J]. Environmental Science , 2018 , 39 ( 3 ): 1278 - 1285 .

Eldyasti A , Nakhla G , Zhu J . Influence of biofilm thickness on nitrous oxide (N 2 O) missions from denitrifying fluidized bed bioreactors (DFBBRs) [J]. Journal of Biotechnology , 2014 , 192 : 281 - 290 .

Gong Y , Peng Y , Yang Q , et al . Formation of nitrous oxide in a gradient of oxygenation and nitrogen loading rate during denitrification of nitrite and nitrate [J]. Journal of Hazardous Materials , 2012 , 227/228 : 453 - 460 .

刘越 , 李鹏章 , 彭永臻 . 短程硝化过程中硝化速率与N 2 O产生速率的关系 [J]. 化工学报 , 2015 , 66 ( 11 ): 4652 - 4660 .

Liu Y , Li P Z , Peng Y Z . Relationship between N 2 O production rate and ammonia oxidation rate during nitritation process [J]. CIESC Journal , 2015 , 66 ( 11 ): 4652 - 4660 .

Peng L , Ni B J , Erler D , et al . The effect of dissolved oxygen on N 2 O production by ammonia-oxidizing bacteria in an enriched nitrifying sludge [J]. Water Research , 2014 , 66 ( 1 ): 12 - 21 .

He Q , Zhu Y , Fan L , et al . Effects of C/N ratio on nitrous oxide production from nitrification in a laboratory scale biological aerated filter reactor [J]. Water Science & Technology , 2017 , 75 ( 5/6 ): 1270 - 1280 .

巩有奎 , 任丽芳 , 彭永臻 . 不同DO下SBBR亚硝酸型同步脱氮及N 2 O释放特性 [J]. 化工学报 , 2019 , 70 ( 4 ): 1550 - 1558 .

Gong Y K , Ren L F , Peng Y Z . Characteristics of simultaneous nitrification and denitrification via nitrite and N 2 O emission in SBBR under different DO concentrations [J]. CIESC Journal , 2019 , 70 ( 4 ): 1550 - 1558 .

He Q , Zhang W , Zhang S . Enhanced nitrogen removal in an aerobic granular sequencing batch reactor performing simultaneous nitrification, endogenous denitrification and phosphorus removal with low superficial gas velocity [J]. Chemical Engineering Journal , 2017 , 326 ( 15 ): 1223 - 1231 .

Guisasola A , Jubany I , Baeza J A , et al . Respirometric estimation of the oxygen affinity constants for biological ammonia and nitrite oxidation [J]. Journal of Chemical Technology & Biotechnology , 2005 , 80 ( 4 ): 388 - 396 .

Soliman M , Eldyasti A . Ammonia-oxidizing bacteria(AOB): opportunities and applications—a review [J]. Reviews in Environmental Science and Bio/Technology , 2018 , 17 ( 2 ): 285 - 321 .

Ma W , Han Y , Ma W , et al . Enhanced nitrogen removal from coal gasification wastewater by simultaneous nitrification and denitrification (SND) in an oxygen-limited aeration sequencing batch biofilm reactor [J]. Bioresource Technology , 2017 , 44 ( 11 ): 84 - 91 .

Sabba F , Picioreanu C , Perez J , et al . Hydroxylamine diffusion can enhance N 2 O emissions in nitrifying biofilms: a modeling study [J]. Environmental Science &Technology , 2015 , 49 ( 3 ): 1486 - 1494 .

Liu Y , Tay J H . The essential role of hydrodynamic shear force in the formation of biofilm and granular sludge [J]. Water Research , 2002 , 36 : 1653 - 1665 .

Fletcher M , Floodgate G D . An electron-microscopic demonstration of an acid polysaccharide involved in the adhesion of a marine bacterium on solid surface [J]. J. Gen. Microbiol ., 1973 , 74 : 325 - 334 .

Tsuneda S , Park S , Hayashi H , et al . Enhancement of nitrifying biofilm formation using selected EPS produced by heterotrophic bacteria [J]. Water Science & Technology , 2001 , 43 ( 6 ): 197 - 204 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

不同C/N下SBBR脱氮过程N2O释放及胞外多聚物变化

运行方式对SBBR亚硝酸型同步脱氮及N2O释放的影响

不同DO下SBBR亚硝酸型同步脱氮及N2O释放特性

MABR中泥-膜复合系统的协同脱氮机制与低碳运行特性研究

厌氧-限氧SBR处理低C/N生活污水SNDPR启动及N2O释放

曝气强度对SBBR同步生物脱氮及N2O释放影响

Effect of aeration intensity on simultaneous biological nitrogen removal and N2O release from SBBR

DOI：10.11949/0438-1157.20190522

摘要

Abstract

关键词

Keywords

references

曝气强度对SBBR同步生物脱氮及N₂O释放影响

Effect of aeration intensity on simultaneous biological nitrogen removal and N₂O release from SBBR