Laccase from Coriolus versicolor was immobilized by adsorbing first on activated carbon

and then entrapping into calcium alginate gels. 2

4-Dichlorophenol in wastewater was dechlorinated by the immobilized laccase in a columnar reactor. The optimal temperature and pH for the reaction were 40℃ and 4.5

respectively. Compared with free laccase

the immobilized laccase had better thermostability

and also had a broader active range of pH and temperature. The decholorination efficiency of laccase on 2

4-dichlorophenol (1 mmol8226;L-1) was all above 99.5% with 0.5 mmol8226;L-1 chloride ions released into the water during 8 repeated batch reactions. The results showed good prospect of application in the biochemical treatment of polluted wastewater.