ZHOU Xin, SUN Yan, LIU Zheng. Preparation of macro-porous pellicular agarose-glass composite matrix and its application to protein adsorption[J]. CIESC Journal, 2006, 57(2).
ZHOU Xin, SUN Yan, LIU Zheng. Preparation of macro-porous pellicular agarose-glass composite matrix and its application to protein adsorption[J]. CIESC Journal, 2006, 57(2).DOI:
A macro-porous pellicular agarose-glass composite matrix(MPAG)was prepared by coating 6% agarose gel onto glass beads by water-in-oil emulsification using a mixture of cyclohexane and fine calcium carbonate powder as porogenic diluents. The density of MPAG was 1.7 g8226;ml-1 and the average bead size was 127.8 μm. MPAG was derivatized with diethylaminoethyl(DEAE) and subjected to the adsorption of bovine serum albumin. Compared to macro-porous matrix or homogeneous matrix derivatized with DEAE
DEAD-MPAG gave a higher adsorption capacity at a faster mass transfer rate.