This paper describes the generation of monodisperse fluorescence aerosols for instrument calibration study. Important considerations in the use of these aerosols are discussed. These include solution preparation

monodisperse aerosol generation

material extraction and analysis. A comparison of two commonly used materials

i. e.

uranine (sodium salt of fluorescein) and Rbodamine B was made. It was found that uranine is most suitable to be used because of its higher detection sensitivity and higher solubility in the solvent. The use of these aerosols in the wind tunnel and laminar flow dust chamber have been described. The technique has been applied to the evaluation of a new type of an axial cyclone. Detailed information concerning particle precipitation in the cyclone is given. It was found that the collection efficiency of the cyclone is a function of the Stokes number.