The influence of L-proline on the electrochemical oxidation of adrenaline was investigated by cyclic voltammetry and electrochemical impedance spectroscopy at a platinum electrode.Simultaneously the structure of complexes of protonated adrenaline with proline is optimized by means of the density function theory method at B3LYP/6-31++G（2 d）level.The experimental results show that proline has stabilization on adrenaline and to some extent inhibits the oxidation of adrenaline.Cyclic voltammetry display that oxidation peak potential shift positively，the reduction peak potential to shift negatively，peak currents decreased.Nyquist plot showed that the electrochemical reaction resistance is accreted.The calculation results indicated that stronger hydrogen bond complexes were formed by the interaction between adrenaline and proline.