By using genistein and calcium hydroxide as raw materials

calcium-genistein complex was synthesized and characterized with IR spectra

UV-spectra

thermal analysis and NMR spectrum. The biological activities of the complex were studied by means of pyrogallol autoxidation

Fenton reaction

orthophenanthroline photometry and DPPH free radical. And also the bacteriostasis activities of the ligand and its complex against three different types of bacteria

namely: Escherichia coli，Pseudomonas aeruginosa，Listeria monocytogenes were studied.  The results showed that in calcium-genistein complex Ca2+ was coordinated by 5-OH and 4-CO groups of genistein and the molecular formula was［Ca(C15H9O5)2］·2H2O. At the same concentration

the scavenging effects of Gen-Ca on superoxide radical and anti-hydroxy free radical showed better activity than the free ligand

but anti-DPPH free radical ability was not so obvious. The ligand and its complex had certain bacteriostasis effect

and the complex showed increased activity compared to the respective ligand.