In order to obtain lactic acid bacteria using molasses wastewater to produce lactic acid，an activated sludge was sampled from bioreactor with molasses wastewater，and strain MD-9 was isolated by an anaerobic Hungate technique. The morphology，physic-biochemical characteristics and 16S rRNA sequence analysis of strain MD-9 were investigated. The results indicated that 16S rRNA gene homology of strain MD-9 with Lactobacillus fermentum was 99. 6%.Strain MD-9 is facultative anaerobe bacillus. Glucose fermentation is heterologous. Strain MD-9 can use molasses wastewater to produce lactic acid.