ZHOU Jun, XU Hong, WANG Jun, et al. Purification and structural characterization of ε-PL produced by Kitasatospora sp.PL6-3[J]. CIESC Journal, 2006, (8).
ZHOU Jun, XU Hong, WANG Jun, et al. Purification and structural characterization of ε-PL produced by Kitasatospora sp.PL6-3[J]. CIESC Journal, 2006, (8).DOI:
ε-Poly-L-lysine(ε-PL) was isolated and purified from the culture broth of Kitasatospora sp.PL6-3.Through the ion exchange process and Sephadex G-25 gel filtration chromatography
a high purity product was obtained with overall yield of 81.2%.The purified ε-PL was characterized with UV
IR and HR/MAS NMR.Its relative molecular weights as determined by SDS-PAGE and gel permeation chromatography (GPC) were about 5.01×103 and 5.05×103 respectively
which was higher than the other ε-PL reported before.