ZHANG Changping, LI Chun, YUAN Yingjin. EFFECTS OF FUNGAL ELICITOR ON SECONDARY METABOLISM OF CELL SUSPENSION CULTURE OF Taxus chinensis var. mairei[J]. CIESC Journal, 2002, (5).
ZHANG Changping, LI Chun, YUAN Yingjin. EFFECTS OF FUNGAL ELICITOR ON SECONDARY METABOLISM OF CELL SUSPENSION CULTURE OF Taxus chinensis var. mairei[J]. CIESC Journal, 2002, (5).DOI:
真菌诱导子对悬浮培养南方红豆杉细胞次生代谢的影响
摘要
在悬浮培养的南方红豆杉细胞体系中
细胞进入指数生长期末期时
加入真菌诱导子 (即尖孢镰刀菌胞壁成分的粗提出物
其主要成分为糖类和多肽 )
对几个主要的细胞次生代谢指标进行了监测 .实验结果表明
诱导子引起了南方红豆杉细胞次生代谢结构的变化
胞内酶蛋白的含量呈先上升后下降的趋势
而胞外酶蛋白的含量显著上升 ;苯丙烷类代谢途径中重要的酶PAL的活性显著升高 ;次生代谢产物中
酚类物质的含量增加
且于 4天后急速上升
同时多酚氧化酶的活力也加强
萜类产物中紫杉烷类的生物合成均得到了加强
其中紫杉醇的含量得到了大幅度的提高
达到对照组的 5倍左右 .
Abstract
Several indexes of metabolism were monitored when the Fungal elicitor originated from Fusarirm oxysporum
whose main ingredients were polysaccharide and protein
was added into the cells suspension cultures system of Taxus chinensis var. mairei in the later exponential growth stage of cells. The results showed that the structure of metabolism was modified
the intracellular protein increased first following a decrease in the later stage
while the extracellular protein kept increasing all the time. PAL
an important enzyme of phenylpropanoid pathway
had its activity promoted obviously
and the phenolics accumulation was strengthened and a sharp increase of it could be observed in the later stage of treatment. At the same time
the activity of PPO increased
and the production of taxol was promoted evidently.