Human epidermal growth factor(hEGF)

a polypeptide growth factor comprising of 53 amino acid residues

has become one of research hot spots in medical field because of its various biological functions. In order to purify hEGF

the influences of flow rate

sample volume and column height on separation performance of hEGF in gel chromatography are investigated. Results indicate that there is a little influence of flow rate on distribution coefficient of hEGF and distribution coefficient of hEGF apparently increases with the increase of sample volume injected

while column height has no influence on distribution coefficient of hEGF. The standard deviation of hEGF decreases with increasing flow rate and column height

especially when flow rate is less than 6cm·h -1 and column height is lower than 8cm. It also increases with the increase of sample volume injected

especially when the sample volume injected is larger than 3ml. These results imply that the resolution will sharply decrease when flow rate and column bed height are less than 6cm·h -1 and 8cm respectively and the sample volume injected is more than 3ml under given conditions.