Mei lehe, Lin Dongqiang, Zhu Ziqiang(Dept.of Biochem.Eng.. SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION[J]. CIESC Journal, 1998, (4).
Mei lehe, Lin Dongqiang, Zhu Ziqiang(Dept.of Biochem.Eng.. SEPARATION OF GLUTATHIONE BY AQUEOUS TWO-PHASE EXTRACTION COMBINED WITH TEMPERATURE-INDUCED PHASE SEPARATION[J]. CIESC Journal, 1998, (4).DOI:
The techniques for separation of glutathione(GSH)by aqueous two-phase extraction combined with temperature-induced phase separation were studied.The partition behavior of GSH in ethylene oxide-propylene oxide random copolymer(EOPO)/hydroxypropyl starch(PES)/water systems was investigated.The effects of such factors
as concentration of polymer
pH
on the partition coefficients in two separation processes(aqueous two-phase extraction and temperature-induced phase separation)were studied.The optimum conditions were EOPO4000 13%
PES100 10%
pH=10.5. The separation technique for GSH from yeast cell was also investigated.A feasible separation scheme was suggested
and the results show that the total extraction yield could reach over 80%.