Dong Xiaoyan, Sun Yan, Yu Kai, et al. TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER[J]. CIESC Journal, 1996, (2).
Dong Xiaoyan, Sun Yan, Yu Kai, et al. TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER[J]. CIESC Journal, 1996, (2).DOI:
A polymerized liposome (PLS) was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions
ligand immobilization
affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg~(-1). These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.