The removal of yeast cell debris following high - speed bead mill disruption was studied by using polyethylene glycol (PEG) precipitation. The partition rule of cell debris was investigated in PEG/inorganic salts systems. Cell debris might be partitioned between the bottom

intermediate and top phases in different aqueous two - phase compositions. When there was only PEG or there were PEG and a little amount of salt

yeast homogenate was separated into a supernatant containing intracellular products and a solid cell debris sediment by using only low speed centrifugation. The effective removal of cell debris and the extraction of intracellular alcohol dehydrogenase were further studied by using the liquid -solid two -phase system and aqueous two -phase systems.