The technical aspects of a new and powerful separation technique

extractive purification by aqueous two-phase systems were studied in detail. According to the characteristics of aqueous two phase systems

a φ20 cylindrical centrifugal extractor was selected as the equipment for extractive purification of α-amylase. The hydraulic characteristics of the installation and the effects of speed and total throughput on the yield and Murphree efficiency of α-amylase extraction were investigated. A speed of 3380-3800r/min

a flow ratio of 0.2-0.5 and a total throughput of 25-50ml/min were found as the optimal operation range for while 90% of phases purity

75% yield and 90% Murphree efficiency for a-amylase could be obtained.