Based on the optimization of culture conditions for producing 2-keto-D-gluconic acid(2-KDG)by Serratia sp.BK-98 in a Erlenmeyer flask

the factors of dissolved oxygen(DO)and pH affecting 2-KDG batch fermentation in 100 L fermenter were further optimized to be 30% and 6.0 respectively.Under the DO-stat and pH-stat batch culture conditions

2-KDG production reached 211.2 g·L-1.The kinetics of DO-stat and pH-stat batch fermentation were also investigated and the models for biomass

substrate consumption and product were established respectively based on the Logistic equation

Leudeking-Piret equation and Modified Leudeking-Piret equation.Curve fittings for the above models by using experimental data were performed by the non-linear least squares method with the software Origin 8.0.With the evaluated model parameters

the calculated values of the models and experimental data were in good agreement and the models could provide guidance for 2-KDG fermentation production.