Silica support materials with different and uniform pore size were fabricated by ordered-packing of monodisperse SiO2 spheres through evaporation assembling method.The pore structure was characterized with low temperature N2 adsorption-desorption method，mercury porosimeter and SEM.The results showed that the SiO2 spheres were closely and orderly packed with face centered cubic packing structure in the silica materials with uniform pore sizes.This method is especially appropriate for the assembling of small size（D<300 nm)SiO2 microspheres，and the assembly time is about 7 d.It is found that the uniformity of pore sizes of silica materials is affected by assembly condition and the optimal temperature of assembling opal is 50℃.