(（S）-（-）-β-hydroxy-benzenepropanoic acid ethyl ester was synthesized by asymmetric reduction of β-carbonyl phenylpropionate with immobilized CGMCC No.2266 in dibutyl phthalate.100 ml microbial liquor containing 430 mg（dry weight）biomass preheated at 50℃ for 30 min was immobilized.After re-culturing for 72 h，the immobilized cells of 2 mm diameter prepared by 2% sodium alginate was used to reduce 17.2 mmol·L-1 β-carbonyl phenylpropionate in 300 ml dibutyl phthalate.The yield and enantiomeric excess of（S）-（-）-β-hydroxy-benzenepropanoic acid ethyl ester reached 92.2% and 100% respectively.Immobilized CGMCC No.2266 can be reused in reaction for ten times.The yield decreased with increasing substrate concentration.Batch addition of substrate can reduce inhibition of high concentration substrate.