By using the molecular imprinting technology，the novel Pb（Ⅱ）imprinting chitosan-coated diatomite beads with Pb（Ⅱ）as imprinted ions（PbCSDE）were prepared.The adsorption behavior for heavy metal ions from water-extraction liquid of Rhodiola L.was studied.The effects on adsorption ability of such factors as adsorption time，pH value of the initial solution were investigated.Adsorption ability for lead and copper ions of PbCSDE was 2 times higher than that of 732 resins.Moreover，the influence of adsorption on salidroside was analyzed by the UV spectra and HPLC.The results revealed that the salidroside in the water-extraction liquid of Rhodiola L.did not change after adsorption by PbCSDE.