Rhodococcus sp.DS-3 with the ability to selectively desulfurize dibenzothiophene and grow with the sulfur atom desulfurized was quantitatively characterized in batch cultures.The fermentation process for desulphurization by DS-3 was analyzed.The transformation of dibenzothiophene to 2-hydroxydiphenyl was 86.3% in BioStat C10-3 fermentor with aerating at 48 L8226;h-1

constant pH 7.8

fermentation temperature 30℃ and stirring at 300 r8226;min-1.The kinetic models of growth，substrate consumption and product generation were established.The optimal parameters were estimated and fitted to model software MATLAB.The results

which showed the actual values in 95% confidence interval

proved that these kinetic models fitted well and fundamentally reflected the regularity of dibenzothiophene desulphurization in the fermentor.